[英文原版 图形算法] microarray image analysis：an algorithmic approach：3. data services

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1、3DataServices3.1IntroductionChapter2discussedthebiologicalconceptsthatmicroarraysexploitfortheirusage.Thesebiologicalmechanismswereexplainedinbroadtermsalongwithadetaileddescriptionoftheirtechnologicalmanifestationssuchthatanun-derstandingofthebasicsof

2、complementarydeoxyribonucleicacid(cDNA)microarraysystemsisclarified.AsdiscussedinSection2.5,ourproposedCo-paseticMicroarrayAnalysis(CMA)frameworkattemptstooperateinatrulyblindmanner.Thismeans(excludingthefailsafemeasuresoftheImageLayout(IL)andImageStruc

3、ture(IS)components)theframeworkoperateswithouttheneedofmanualassistance.TheframeworkshouldperformtheanalysistaskssuchthattheyarecomparabletotheGenePixtypeprocessasdiscussedinSection2.4.However,duetothehighsignalvariabilityseenacrossthetestdataset(whenw

4、orkingdirectlywiththerawmicroarrayimagery),identify-inggenespotscanbecounterproductiveatthisearlystage.Therefore,ratherthanusetherawimage,itissuggestedthatproducingviewsoftheimagedatasuchthatemphasisisplacedoncertainfrequenciesorregionsofinterestwouldn

5、otonlybeadvantageous,butmoreeffectiveintermsoftheoverallgoal.Thismulti-viewanalysisprocessismanagedbytheapplicationoftheImageTransformationEngine(ITE)componenttothemicroarrayimagedata.Inthecurrentimplementation,theITEcomponentgeneratesanimagedesignedtoe

6、nhancethepositionsofthegenespots.Althoughthispositionalinformationispartialinsomeareasofthenewlygeneratedimagesurface,itisfitforidentificationpurposesatthisstage.AswashighlightedinChapter2theCMAframework’sprocessesarede-signedsuchthattherequirementsofman

7、ualinterventionareminimized.Thisdesignconstraintcreatesanon-trivialsearchproblemhowever.Themicroar-rayimagesareembeddedwithgenespotsfortheparticularexperimentalrunasrequired.However,duetothenatureofthebiologicalprocessandthede-siredcaveatofnopriordomai

8、nknowledge,thegenespotpositionsmustbedeterminedbyanalgorithmicmethod.Inorderforthegenespotaddressingstagetoreturnappropriateresults,therawmicroarrayimageshouldfirstbepre-processedtoremoveasmuchartifactnoiseaspossible.Thenoiseremoval5354M