[英文原版 图形算法] microarray image analysis：an algorithmic approach：5. structure extrapolation i

《[英文原版 图形算法] microarray image analysis：an algorithmic approach：5. structure extrapolation i》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、5StructureExtrapolationII5.1IntroductionTheresearchinChapter4identifiedproblemsinvolvedwhenattemptingtoap-plytraditionalclusteringmethodstotheanalysisoffullsizecomplementarydeoxyribonucleicacid(cDNA)microarrayimages.Theseproblemsincludedsuchissuesascomputationalcomplexity,lossofimageinform

2、ationanddatablindness.WiththeapplicationofthePyramidicContextualClustering(PCC)andImageTransformationEngine(ITE)componentstotheimagedata,theseissueshavebeenlargelyresolved.Thatistosay,computationalcomplexityanddatalossissueshavebeensignificantlyreducedintimeandseverityre-spectively.Thedata

3、blindness(nopriordomainknowledge)issuewasalsofollowedduringtheexecutionofthesecomponents,withtheindividualcom-ponentresultscapturinganimage’sgenespotdetailverywell.Suchdetailedgenespotinformationmakesfeasibletheacquisitionofagivenimage’struegeneblockstructure.AlthoughneitheroftheITEorPCCg

4、eneratedresultscapturetheentiregenespotsregionsinanimage(exceptinthetrivialcaseofaverycleanimagesurface),theirindividualresultscanbecombinedtosignificantlyenhancethegenespotidentificationprocess.AsdetailedinSection2.5acDNAmicroarrayimageconsistsofseveralmasterblockspropagatedacrossitssurfac

5、e,whichcanalsobeseeninFigure5.2.Inanidealsituation,theseblockswillallbeinperfectalignmentwitheachotherintheverticalandhorizontaldirections,bothinternallytotheblockandexternallyacrosstheslide.Therewillbenorotationorskewwithrespecttotheirglobalpositioning.Theywillnotoverlapeachotherorbesocl

6、oseastoconfusetheiridentificationatalaterstage.Also,internallythemeta-blockswillnotsufferfromanymissinggenespots.Ifthegenespotsaremissingduetotheirintensitybeingweak,theycouldbeobscuredbylocalbackground.However,inpracticetheperfectalignmentofblockswithinanimageisrarelythecase.Masterblocksar

7、enotonlymisalignedwithrespecttoeachother;theyalsotendtohaverotationissues.Inthecasewherethereislargerotationinthesurface,thesemasterblockscanbedifficulttoacquireaccu-rately(dependingontheidentificationprocessinvolved).Typicallythough,theserotationstendtobesmallandasare