紫杉醇对LASEK术后角膜Haze形成影响的分析

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1、万方数据中文摘要组和MMC组组织结构均趋于正常，连续并较完整。而BSS组术区中央角膜上皮细胞增生明显，基底细胞大小不一，胶原纤维增生、排列紊乱明显，纤维间隙较大，而术后角膜基质层qUTGF．fll的表达水平随时间延长而成下降趋势。3角膜超微结构的观察：与丝裂霉素组和BSS组相比，紫杉醇组术后角膜前基质有较少的成纤维细胞，角膜基质层胶原纤维直径相近，排列规则，成纤维细胞体积小，细胞器也不多，状态稳定。结论：紫杉醇能够显著减少LASEK术后兔眼早期角膜基质层TGF．B1的表达水平，从而对角膜haze的形成有明显的抑制作用，其疗效与

2、丝裂霉素相同，可减少纤维化反应，形成较少的角膜Haze，使角膜保持透明。本研究结果提示了紫杉醇应用于防治LASEK术后角膜haze的可行性和临床意义。关键词：紫杉醇，准分子激光上皮下角膜磨镶术，角膜上皮下雾状混浊，转化生长因子．B1，丝裂霉素万方数据英文摘要StudyOilpaclitaxelinhibitinghazeafterLASEKinrabbitsABSTRACTObjective：Toinvestigatetheinhibitingeffectsofpaclitaxel(tax01)onhazeaftersubep

3、ithelialkeratomileusis(LASEK)inrabbits．Methods：1ToestablishrabbitLASEKmodel，thirty—sixhealthyNewZealandwhiterabbits，afterexclusionofeyediseases，wereundergone一10．0diopterLASEKoperation．2．Allrabbitswereequallydividedintothreegroupsinrandomizedmethod．byself-controlmode

4、。Group0．3mg／mLpaclitaxeltreatinggroupduringoperation；Group2：0．02％MMCtreatinggroupduringoperation；Group3：Balancesalinesolution(Bss)treatinggroupduringoperation，ascontrolgroup12rabbitsineachgroup．Partt：Torandomlyselect4rabbits(8eyes)ineachgroup，嘲larlyobservecornealepi

5、theliumhealingprocessandocularsigns．assesstheextentofhazebyslitlampmicroscope，when1week，lmonthand3montllSafteroperation．Part2：Cornealepitheliumhistopathologicexamination，transmissionelectronmicroscopeobservationandimmunohistochemistryexaminationtestingTGF一91wereappl

6、iedwhen1week，lmonthand3monthsafteroperation．Results：1EvaluationbyslitlampmicroscopeafterLASEK：comealepithelialtissueofallthreegroupswererecoveredinoneweekafterLASEK，andtherewasnosignificantdifferenceamongthethreegroups．After1week，1month，3months，thedifferencesweresta

7、tisticallysignificantp<0．01)amongthethreegroupsonHazedegree，andthepaclitaxelandmitomycingroupwerelowerthanBSSgroup俨0．05)；nosignificantdifference妒>0．05)am

8、ongthethreegroupsafter万方数据英文摘要months．2Cornealepitheliumhistopathologicobservation：Thicknessofcornealepitheliaandepitheliallayersobviouslyi