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ID:39144783
大小:2.40 MB
页数:37页
时间:2019-06-25
《紫杉醇对LASEK术后角膜Haze形成影响的分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据中文摘要组和MMC组组织结构均趋于正常,连续并较完整。而BSS组术区中央角膜上皮细胞增生明显,基底细胞大小不一,胶原纤维增生、排列紊乱明显,纤维间隙较大,而术后角膜基质层qUTGF.fll的表达水平随时间延长而成下降趋势。3角膜超微结构的观察:与丝裂霉素组和BSS组相比,紫杉醇组术后角膜前基质有较少的成纤维细胞,角膜基质层胶原纤维直径相近,排列规则,成纤维细胞体积小,细胞器也不多,状态稳定。结论:紫杉醇能够显著减少LASEK术后兔眼早期角膜基质层TGF.B1的表达水平,从而对角膜haze的形成有明显的抑制作用,其疗效与
2、丝裂霉素相同,可减少纤维化反应,形成较少的角膜Haze,使角膜保持透明。本研究结果提示了紫杉醇应用于防治LASEK术后角膜haze的可行性和临床意义。关键词:紫杉醇,准分子激光上皮下角膜磨镶术,角膜上皮下雾状混浊,转化生长因子.B1,丝裂霉素万方数据英文摘要StudyOilpaclitaxelinhibitinghazeafterLASEKinrabbitsABSTRACTObjective:Toinvestigatetheinhibitingeffectsofpaclitaxel(tax01)onhazeaftersubep
3、ithelialkeratomileusis(LASEK)inrabbits.Methods:1ToestablishrabbitLASEKmodel,thirty—sixhealthyNewZealandwhiterabbits,afterexclusionofeyediseases,wereundergone一10.0diopterLASEKoperation.2.Allrabbitswereequallydividedintothreegroupsinrandomizedmethod.byself-controlmode
4、。Group0.3mg/mLpaclitaxeltreatinggroupduringoperation;Group2:0.02%MMCtreatinggroupduringoperation;Group3:Balancesalinesolution(Bss)treatinggroupduringoperation,ascontrolgroup12rabbitsineachgroup.Partt:Torandomlyselect4rabbits(8eyes)ineachgroup,嘲larlyobservecornealepi
5、theliumhealingprocessandocularsigns.assesstheextentofhazebyslitlampmicroscope,when1week,lmonthand3montllSafteroperation.Part2:Cornealepitheliumhistopathologicexamination,transmissionelectronmicroscopeobservationandimmunohistochemistryexaminationtestingTGF一91wereappl
6、iedwhen1week,lmonthand3monthsafteroperation.Results:1EvaluationbyslitlampmicroscopeafterLASEK:comealepithelialtissueofallthreegroupswererecoveredinoneweekafterLASEK,andtherewasnosignificantdifferenceamongthethreegroups.After1week,1month,3months,thedifferencesweresta
7、tisticallysignificantp<0.01)amongthethreegroupsonHazedegree,andthepaclitaxelandmitomycingroupwerelowerthanBSSgroup俨0.05);nosignificantdifference妒>0.05)am
8、ongthethreegroupsafter万方数据英文摘要months.2Cornealepitheliumhistopathologicobservation:Thicknessofcornealepitheliaandepitheliallayersobviouslyi
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