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《Correlating Cell Cycle With Metabolism in Single CellsCombination of Image and Metabolic Cytometry细胞周期与单细胞代谢相关： 意象与Metabolic Cytometry的结合》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、r1999Wiley-Liss,Inc.Cytometry37:14±20(1999)CorrelatingCellCycleWithMetabolisminSingleCells:CombinationofImageandMetabolicCytometrySergeyN.Krylov,ZheruZhang,NoraW.C.Chan,EdgarArriaga,MonicaM.Palcic,andNormanJ.Dovichi*DepartmentofChemistry,UniversityofAlberta,Edmonto
2、n,Alberta,CanadaReceived5March1999;RevisionReceived4May1999;Accepted4May1999Background:Wecointwoterms:First,chemicalcytome-separatedbycapillaryelectrophoresisanddetectedbytrydescribestheuseofhigh-sensitivitychemicalanalysislaser-induced¯uorescence.techniquestostudy
3、singlecells.Second,metaboliccytom-Results:Substrateuptakemeasuredwithmetaboliccytom-etryisaformofchemicalcytometrythatmonitorsaetrygaverisetoresultssimilartothosemeasuredbyuseofcascadeofbiosyntheticandbiodegradationproductsgen-laserscanningconfocalmicroscopy.TheDNA
4、ploidyeratedinasinglecell.Inthispaper,wedescribethehistogramobtainedwithoursimpleimagecytometrycombinationofmetaboliccytometrywithimagecytome-techniquewassimilartothatobtainedusing¯owcytome-trytocorrelateoligosaccharidemetabolicactivitywithcelltry.ThecellsintheG1ph
5、asedidnotshowanybiosynthe-cycle.WeusethistechniquetomeasureDNAploidy,theticactivityinrespecttothesubstrate.Severalgroupsofuptakeofa¯uorescentdisaccharide,andtheamountofcellswithuniquebiosyntheticpatternsweredistinguishedmetabolicproductsinasinglecell.withinG2/Mcell
6、s.Methods:Acolonadenocarcinomacellline(HT29)wasConclusions:Thisisthe®rstreportthatcombinedincubatedwitha¯uorescentdisaccharide,whichwasmetabolicandimagecytometrytocorrelateformationoftakenupbythecellsandconvertedintoaseriesofmetabolicproductswithcellcycle.Acomplete
7、enzymaticbiosyntheticandbiodegradationproducts.Thecellswerecascadeismonitoredonacell-by-cellbasisandcorrelatedalsotreatedwithYOYO-3andHoechst33342.TheYOYO-3withcellcycle.Cytometry37:14±20,1999.signalwasusedasalive-deadassay,whiletheHoechstr1999Wiley-Liss,Inc.33342s
8、ignalwasusedtoestimatetheploidyoflivecellsby¯uorescenceimagecytometry.Afterploidyanalysis,acellwasinjectedintoafused-silicacapillary,wherethecell
