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1、生物工程学报ChinJBiotech2008,June25;24(6):1022-1028journals.im.ac.cnChineseJournalofBiotechnologyISSN1000-3061cjb@im.ac.cn©2008InstituteofMicrobiology,CAS&CSM,Allrightsreserved研究报告海洋野生鱼酶解提取鱼油的工艺分析钱俊青,单昱东,廖启元浙江工业大学药学院,杭州310014摘要:海洋野生鱼与养殖鱼比较,其鱼油中含更多的二十碳五烯酸（EPA）、二十二碳六烯酸（DHA）、脂溶性维生素等活性成分。为提高海洋野生鱼的利用价值,以野生
2、小带鱼为原料进行酶法提油工艺研究。分析了不同的温度,时间,pH值等影响因素下的提取、萃取以及离心效果,以响应面法确定了最佳的酶解工艺条件:液固比为6、pH7.3、酶量1000oou/g原料、搅拌速度200r/min、45C酶解90min;最优萃取条件:萃取剂100mL(每20g鱼糜原料)、pH4.0、40C萃取25min;离心条件:离心速度3000r/min(1865g)、离心时间10min。上述工艺条件下提油率为79.90%。改进了传统的鱼油提取工艺,在活性成分保护上有较大改善。关键词:海洋野生鱼,鱼油,酶解,萃取,离心,工艺TechnologyInvestigationofExtr
3、actingFishOilbyEnzymolysisingOceanWildFishJunqingQian,YudongShan,andQiyuanLiaoCollegeofPharmaceuticalScience,ZhejiangUniversityofTechnology,Hangzhou310014,ChinaAbstract:Comparedwithculturedfish,thefishoilofoceanwildfishcontainsmuchmoreEicosapntemacniocacid(EPA),Docosahexenoicacid(DHA),fat-solubl
4、evitamin.Toimprovetheutilityvalueofoaceanwildfish,smallhairtailwasusedasrawmaterialtoinvestigatethetechnologyofextractingfishoilwithenzyme.Thevariablestoaffecttheefficiencyofextraction,extractingandcentrifugationwereselectedastemperature,reactiontimeandpHvalue.Optimaltechnologyconditionsweredete
5、rminedbytheresponsesurfacemethod:Theliquid/solidratiois6,pH7.3,enzymeamountof1000u/grawmaterial,agitationospeedof200r/min,enzymolysisunder45Cfor90min.Theoptimumextractionconditionswereasfollows:100mLextractanto(every20gsurimi),pH4.0,extractedunder40Cfor25min.Theoptimalcentrifugeconditionswere:ce
6、ntrifugespeedof3000r/min(1865g),centrifugedfor10min.Theoilextractionefficiencywas79.9%.Thisstudydevelopedthetraditionaltechnologyoffishoilextraction,andimprovedtheprotectionoftheactivecomponents.Keywords:oceanwildfish,fishoil,enzymolysis,extraction,centrifuge,technology[8][9−10][11]鱼油中富含EPA、DHA、
7、脂溶性维生素及其他抗炎、抗风湿;健脑增智;增强免疫力;保[1][12]成分。EPA和DHA有抗血小板凝聚、延缓血栓形护视力等生理功效。[2][3,4][5−7]成;降血脂和抗动脉硬化;抑制肿瘤生长;随着对鱼油研究的深入,人们越来越认识到鱼Received:March15,2008;Accepted:April10,2008Supportedby:KeyProgramoftheScienceandTechnologyPlanofZhejiangP
