资源描述：
《多药耐药相关蛋白_2在白血病耐药细胞株K562_A02的表达》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、第36卷第2期吉林大学学报(医学版)Vol.36No.23122010年3月JournalofJilinUniversity(MedicineEdition)Mar.2010[文章编号]16712587Ú(2010)0220312204多药耐药相关蛋白22在白血病耐药细胞株K562/A02的表达姜振宇1,许颖2,荆蕾3,姚程3,马宁1(11吉林大学第一医院风湿科,吉林长春130021;21吉林大学第一医院肾病内科,吉林长春130021;31吉林大学第一医院血液肿瘤科,吉林长春130021)[摘要]目的:研究多药耐药相关蛋白22(MRP22)与白血病细胞耐药的关系,并探讨其在白血病多药
2、耐药中的意义。方法:应用RT2PCR方法检测白血病细胞株K562与耐药细胞株K562/A02中MRP22mRNA的差异表达,然后将MRP22反义及正义寡核苷酸片段分别用脂质体转染至耐药细胞株K562/A02,采用MTT法、流式细胞术及RT2PCR法检测转染后细胞内阿霉素的荧光强度、耐药指数及MRP22mRNA的表达情况。结果:耐药细胞株K562/A02中MRP22mRNA表达水平明显高于白血病细胞株K562(P<0105)。MRP22反义寡核苷酸片段转染后耐药细胞株K562/A02细胞内的阿霉素浓度升高,耐药指数较未转染细胞明显降低(P<0105);转染后耐药细胞株K562/A02细
3、胞MRP22mRNA表达水平较未转染细胞下降了6715%,两者比较差异具有显著性(P<0105)。结论:MRP22的高表达导致白血病耐药细胞内化疗药物浓度降低,可能是其导致白血病多药耐药的机制之一。[关键词]白血病;多药耐药;多药耐药相关蛋白22[中图分类号]R73317[文献标志码]AExpressionofmulti2drugresistancerelativeprotein22inleukemiamulti2drugresistancecelllineofK562/A02JIANGZhen2yu1,XUYing2,JINGLei3,YAOCheng3,MANing1(11Dep
4、artmentofRheumatology,FirstHospital,JilinUniversity,Changchun130021,China;21DepartmentofNephrology,FirstHospital,JilinUniversity,Changchun130021,China;31DepartmentofHematologyandOncology,FirstHospital,JilinUniversity,Changchun130021,China)Abstract:ObjectiveToinvestigatetherelationshipbetweenmul
5、ti2drugresistancerelativeprotein22(MRP22)andleukemiamulti2drugresistance,andstudyitseffectonleukemiamulti2drugresistance.MethodsTheexpressionsofMRP22inleukemiacelllineK562andleukemiamulti2drugresistancecelllineK562/A02weredetectedbyPT2PCR.Anti2andoligonucleotideofMRP22weretransfectedbylipidosom
6、eintoK562/A02,intra2cellulardoxorubicin(ADM)fluorescenceintensity,resistanceindex,andtheexpressionofMRP22mRNAweredetectedbyMTT,flowcytometry,andRT2PCR.ResultsTheexpressionofMRP22inK562/A02washigherthanthatofK562(P<0105).Aftertransfectedwithanti2oligonucleotideMRP22,theADMfluorescenceintensityof
7、K562/A02wasincreased,andtheresistanceindexwassignificantlydecreased,andtheexpressionofMRP22mRNAwasdecreasedby6715%(P<0105)comparedwithuntransfectedcells.ConclusionThehigh2levelexpressionofMRP22resultsinthereducingofchemicaldrugcon
