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1、ActaPhysiologicaSinica,ZHENGZhao-Qingetal:DualActionofLPAinCulturedCorticalNeurons:SurvivalandApoptogenicApril25,2004,56(2):163-171163http://www.actaps.com.cnResearchPaperDualactionoflysophosphatidicacidinculturedcorticalneurons:survivalandapoptogenicZHENGZhao-Qing1,FANGXian-Jun2,QIAOJian-T
2、ian1*1DepartmentofNeurobiology,ShanxiMedicalUniversity,Taiyuan,Shanxi030001,China2M.D.AndersonCancerCenter,DepartmentofMolecularTherapeutic,1515HolcombeBlvd.,Houston,TX77030,USAAbstract:Theeffectoflysophosphatidicacid(LPA),withawiderangeofitsdifferentconcentrations,uponculturedmousecortical
3、neuronswasassessedbyelectrophoresisofDNAfragments,HO33342andTUNELstainings,andalsobyultrastructuralexaminationattimes.TheresultsshowedthatadministrationofLPAatlowerconcentrations(0.1~30µmol/L)dose-dependentlyprotectedcorticalneuronsfromapoptosisthatwasinducedbydeprivationofserumfromthecultu
4、ralmedium,while50µmol/LorhigherconcentrationsofLPAfailedtoshowthiseffect;andmoreover,theconcentrationshigherthan50µmol/Linducedapoptosisinneuronsculturedinserum-containingcompletemedium.TheseresultssuggestthatamoderateconcentrationofLPAmayplayasasurvivalfactorinapoptoticcorticalneurons,whil
5、eanexcessivelevelofLPAinducesapoptosisinneuronsculturedincompletemedium.Keywords:lysophosphatidicacid;apoptosis;survivalfactor;serum-freemedium;completemedium;corticalneurons;mice121,*1,030001;2,77030,:DNAHO33342TUNEL,(lysophosphatidicacid,LPA),LPA(0.1∼30µmol/L),LPA(>50µmol/L),,LPA,LPA:;;;;
6、;;:Q421Apoptosis,orprogrammedcelldeath,isanactivecellLysophosphatidicacid(LPA)isthesmallestandstruc-[1]responsetophysiologicalordamagingsignals.Morpho-turallysimplestofallglycerophospholipidspresenting[6,7]logically,apoptosisischaracterizedbycondensationofinserum,andisnormallyreleasedbyacti
7、vatedplate-[7]nuclearchromatin,DNAfragmentation,compactionofcy-letsduringbloodclotting.LPAbindswithhighaffinity[8]toplasmicorganelles,cellshrinkage,andrufflingandtoserumalbuminwhileretainingitsbiologicalactivity.[1,2][9]blebbingoftheplasmamembrane.Cellde
