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《溶血磷脂酸对β-淀粉样蛋白片段aβp31-35所致小鼠大脑皮层离体神经元 凋亡的神经保护作用》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、ActaPhysiologicaSinica,June25,2005,57(3):289-294289http://www.actaps.com.cnResearchPaperNeuroprotectiveeffectoflysophosphatidicacidonAβP31-35-inducedapoptosisinculturedcorticalneuronsZHENGZhao-Qing1,FANGXian-Jun2,ZHANGYu1,QIAOJian-Tian1,*1DepartmentofNeurobiology,ShanxiMedicalUniversity,Taiyuan0
2、30001,China;2VirginiaCommonwealthUniversity,DepartmentofBiochemistry,1101EastMarshallStreet,Richmond,VA23298,USAAbstract:Ithasbeenreportedthatlysophosphatidicacid(LPA)atitslowerconcentrationspreventsapoptosisinducedbyserum-deprivationinculturedcorticalneuronswhenLPAisaddedintotheculturalmediumwi
3、thserumwithdrawal.ThepresentstudywasdesignedtoinvestigatewhetherLPAcouldalsoblocktheapoptosisinducedbyβ-amyloidpeptidefragment31-35(AβP31-35)inculturedcorticalneuronsbyusingtechniquesofDNAfragmentationelectrophoresis,HO33342staining,andTUNELexaminations.TheresultsshowedthatpretreatmentofLPAsuppr
4、essedtheAβP31-35-inducedapoptosisonlywhenLPAwasappliedtotheculturedneuronswithlowerconcentrations(1~10µmol/L)andespecially,withaprecedingtimeof12~24hbeforetheAβP31-35exposure.ThesefactsimplythatLPAalsoactsasaneuroprotectivefactoragainstAβP31-35-inducedapoptosis,thoughthemechanismunder-lyingthepr
5、otectiveactioninthiscasemaybemorecomplexthanthatinvolvedintheserumdeprivation-inducedapoptosis.Keywords:culturedcorticalneurons;apoptosis;β-amyloidpeptidefragment31-35;lysophosphatidicacid;neuroprotectiveeffect;mice溶血磷脂酸对β-淀粉样蛋白片段AβP31-35所致小鼠大脑皮层离体神经元凋亡的神经保护作用郑肇青1，方羡君2，张宇1，乔健天1,*1山西医科大学神经生物学研究室,
6、太原030001；2弗吉尼亚Commonwealth大学生物化学系,Richmond,VA23298,美国摘要:已报道低浓度溶血磷脂酸(lysophosphatidicacid,LPA)对去血清培养所致的神经元凋亡有神经保护作用。为了进一步观察LPA是否对β-amyloidpeptidefragment31-35(AβP31-35)所致的神经元凋亡也起类似的作用,本研究应用DNA电泳分析、HO33342和TUNEL染色法等技术，对培养的小鼠大脑皮层神经元进行了观察。结果显示,只有使用较低浓度的LPA(1~10µmol/L)、并且将此剂量的LPA比AβP31-35提前12~24h加入培养
7、液时，才可看到LPA明显削弱了AβP31-35所致的神经元凋亡。以上结果表明，适当浓度的LPA在长时间预作用的条件下，可对AβP31-35所致的皮层神经元凋亡起保护因子或抗凋亡因子的作用,但其作用途径可能较在去血清培养所致的凋亡时更为复杂，因为在去血清的同时加入LPA就能制止去血清所致的凋亡。关键词:皮层神经元;凋亡;β-淀粉样蛋白31-35;溶血磷脂酸;神经保护作用;小鼠中图分类号:Q421Thestructurallysimplestgly
