超声破坏LHRH靶向微泡联合紫杉醇治疗卵巢癌的实验研究

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1、重庆医科大学硕士学位论文超声破坏LHRH靶向微泡联合紫杉醇治疗卵巢癌的实验研究姓名：贺娟申请学位级别：硕士专业：妇产科指导教师：孙江川20090501重庆医科大学硕士研究生学位论文’关键词：靶向超声造影剂，卵巢癌，LHRH第二部分超声破坏LHRH靶向脂质微泡联合紫杉醇对卵巢癌细胞ovcar3的增殖、凋亡及细胞内药物浓度的影响目的：从细胞增殖和凋亡及细胞内药物浓度，观察靶向微泡协同紫杉醇联合超声辐照对Ovcar3细胞的抑制增殖和诱导凋亡作用和细胞内药物浓度的上调影响。，方法：通过MTT比色法选择紫杉醇对Ovcar3，细胞体外治疗浓度，然后检测靶向微泡协同紫杉

2、醇联合超声辐照后卵巢癌Ovcar3细胞的增殖能力；采用FCM法测诱导凋亡率；采用HPLC法检测靶向微泡协同紫杉醇联合超声辐照对卵巢癌Ovcar3细胞作用后细胞内紫杉醇药物浓度的改变。结果：紫杉醇对Ovcar3细胞体外治疗的有效浓度是10．6mol／L；靶向微泡协同紫杉醇联合超声辐照Ovcar3细胞具有更明显的抗增殖能力和诱导细胞凋亡能力。且使细胞内药物浓度明显增加。结论：TUCA协同紫杉醇联合超声辐照不仅能抑制卵巢癌Ovcar3细胞增殖，还有诱导其凋亡的作用，并能使其细胞内药物浓度上调。关键词：超声，靶向超声造影剂，紫杉醇，卵巢癌，OVCAR3，细胞凋亡，

3、细胞内药物浓度3重庆医科大学硕士研究生学位论文THESTUDYOFULTRASoUNDDESTRUCTIoNLHRHTARGETEDMICRoBUBBLESCoMBINEDPACLITAXELTREATMENToVARIANCANCERABSTRACTPARTIPREPARATIoNANDoBSERVATIoNoFTARGETEDABILITYoFLIPoSoMEMICRoBUBBLESTARGETEDHUMANoVARIANCANCERCELLLINEoVCAR-3INVITRoObjective：Topreparehumanovariancancer-

4、-targetedliposomemicrobubblesandassessitstargetedabilityinvitro．Methods：ligandLHRHwasattachedtothesurfaceofself-madeliposomemicrobubblesbycovalentbondingtopreparetargetedmicrobubbles．ImmunofluorescentstainingassayWasusedtoidentifythecombinationofLHRHwithliposomemicrobubblesandthet

5、argetedmicrobubbleswereaddedtoovariancancercellsovcar-3andthenobservedunderthelightandfluorescencemicroscopetoevaluatethetargetingabilityofthetargetedpaclitaxelliposomemicrobubbleswithovariancancercellsinvitro，whilethecommonliposomemicrobubbleswerecontrols．Anduse4‘重庆医科大学硕士研究生学位论文L

6、HRHtodoablockexperiment．Results：Targetedmicrobubbleswerepositiveinimmunofluorescentstrainingassay．InvitrostudyofthetargetingabilityshowedthetargetedmicrobubblescouldactivelyadheretoOVCar一3cel1．WhilethecontrolWasnegative．AndLHRHcaneffectivelyblockthetargetedmicrobubbles．Conclusion：

7、Thetargetedliposomemicrobubblescanbepreparedsuccessfltllybycovalentbondingmethod，thistargetedmicrobubblescouldbindtohumanovariancancercellsspeciallyandefectivelyinvitro．Keywortls：targetedliposomemicrobubbles；bvariancancer；L职HPARTIIULTRASOUNDDESTRUCTLHRHTARGETEDLIPoSoMEMICRoBUBBLES

8、CoMBINEDPACLITAXELoNoVARIANCARCIN