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ID:36562225
大小:6.13 MB
页数:72页
时间:2019-05-12
《根癌农杆菌介导的ANTICTLA4HS83基因遗传转化甘薯的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、西南交通大学硕士学位论文根癌农杆菌介导的ANTI-CTLA-4-HS83基因遗传转化甘薯的研究姓名:许培芳申请学位级别:硕士专业:生物化学与分子生物学指导教师:李萍;阎文昭20080401西南交通大学硕士研究生学位论文第1l页AbstractPlant-basedexpressionofpharmaceuticalproteinsisacOSt—effectivealtern.ativetotraditionallarge—scaleproductionsystemssuchasmicrobialferment
2、ation,insectandmammaliancellcultures,andtransgenicanimals.Plantsarethemosteconomicalmakersofbiomassusingonlysunlight,water,carbondioxideandminerals.Theyofferapropermodificationsystemforeukaryoticproteins,suchasfolding,post-tr.anslationalmodification,andsub—c
3、ellulartargeting;moreover,theyavoidanimalcell—culturecontaminantssuchasmammalianpmhogensandextraneousviralorbacterialcomponents.Sincethehumangrowthhormonewasexpressedintransgenictobaccoin1986,manymorepharmaceuticallyrelevantproteinshavebeensuccessfullyproduc
4、edinplants.TheproteinsexpressedinplantsCanbeextractedfrom.wholeplantpartsororganellesandusedasasourceformedicinalapplications.Productionofsweetpotato(Ipomoeabatatas(L)Lava.)ranksfifthafterthatofrice,wheat,maizeandwhitepotato,amongthefoodcropsoftheworld.Sweet
5、potatoisoneofthemostimportantcropsfordevelopingcountries,whereitrankssecondaccordingtoitsproductionvalue,andfifthaccordingtoitscaloriecontri--butionforhumans.Sweetpotatohaveahighproductionyieldofbiomass,needlessnutrientsfromthesoilthanmostothercrops.Establis
6、hingsweetpotatoasaproductionsiteforthedeliveryofrecOmbinantproteinsisworthstrivingfor.Singlechainfragmentvariables(scFv)anti—CTLA-hs83isonekindoftargetfragmentsforimmunotoxins,itCallselectivelylinktheactivatedTcells,possessesthefeaturesofspecificity,highacii
7、vityandlowimmunogenicity.Soitmaybehavegoodprospectforpreventionandcureoftransplantrejection.西南交通大学硕士研究生学位论文第1II页Inthisstudy,wedescribeallagrobacteriumbinaryvectorcarryingsinglechainvariablefragment(scFv)anti··CqT.A·-hs83genewassuccessfullyconstructedbasedonp
8、CAMBIAl301,thentransformedsweetpotatoplantswiththisbinaryvectorusingA.tumefaciens-mediatedtransformation,30hygromycinresistantplantswereobtained.Hygromycinresistantplantsweretestedbypolymerasech
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