rhFGF3蛋白在E.coli系统中的表达、纯化及其神经保护作用研究

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1、分类号：R9单位代码：10343学号：151005762硕士学位论文论文题目:rhFGF3蛋白在E.coli系统中的表达、纯化及其神经保护作用研究研究生姓名：赵天天学科专业：药学类型：学术型指导教师：姜潮教授二O一八年五月论文题目:rhFGF3蛋白在E.coli系统中的表达、纯化及其神经保护作用研究答辩委员会主席：蔡跃飘教授答辩委员会成员：廖志勇教授王怡副研究员王萍副教授论文答辩日期：2018年5月20日温州医科大学硕士学位论文目录缩略词表······································

2、·····································1中文摘要···········································································2英文摘要···········································································4前言····························································

3、··················7第一部分rhFGF3载体构建及蛋白诱导表达纯化1引言··············································································92实验仪器与材料·······························································93实验方法···························································

4、············113.1pET3a-hFGF3重组质粒构建·············································113.2重组蛋白的诱导表达·····················································133.3重组蛋白的纯化···························································143.4重组蛋白的纯度测定及保存··························

5、··················144实验结果·······································································144.1重组质粒的鉴定及序列分析·············································154.2重组蛋白的诱导表达······················································154.3重组蛋白的纯化······················

6、······································164.4重组蛋白的纯度测定及保存·············································185分析与讨论····································································18第二部分rhFGF3蛋白的生物学活性研究1引言···························································

7、··················192实验仪器与材料······························································193实验方法·······································································213.1NIH3T3与PC12细胞的培养与保存··································213.2rhFGF3对NIH3T3与PC12细胞的促增殖活性比较·

8、·············223.3脑缺血再灌注细胞模型的建立··········································223.4rhFGF3对OGD/R诱导PC12脑缺血再灌注细胞模型的促增殖作用····································································