FGF9融合蛋白在E.coli系统中的表达与纯化及其亚家族对肝癌细胞增殖和迁移的影响

《FGF9融合蛋白在E.coli系统中的表达与纯化及其亚家族对肝癌细胞增殖和迁移的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、温州医科大学硕士学位论文分类号：R9单位代码：10343学号：151005764硕士学位论文论文题目:FGF9融合蛋白在E.coli系统中的表达与纯化及其亚家族对肝癌细胞增殖和迁移的影响研究生姓名：王申学科专业：药学类型：学术型指导教师：田海山二O一八年五月温州医科大学硕士学位论文论文题目:FGF9融合蛋白在E.coli系统中的表达与纯化及其亚家族对肝癌细胞增殖和迁移的影响答辩委员会主席：廖志勇教授答辩委员会成员：蔡跃飘教授王怡副研究员王萍副教授论文答辩日期：2018年5月20日温州医科大学硕士学位论文目录缩略

2、词表··········································································1中文摘要··········································································3英文摘要··········································································5前言·····················

3、························································7第一部分rhFGF9融合蛋白的表达、纯化和活性测定1引言·············································································92实验仪器和材料······························································93实验方法···················

4、··················································113.1pETM11-His-TEV-hFGF9重组质粒转化至DH5α感受态细胞113.2融合蛋白的诱导表达··················································123.3电泳及Westernblotting检测目的蛋白表达·······················143.4融合蛋白的可溶性检测··································

5、·············163.5融合蛋白的纯化························································163.6融合蛋白的酶切与纯化···············································163.7融合蛋白和非融合蛋白的活性检测································174实验结果························································

6、·············184.1融合蛋白的诱导表达··················································184.2融合蛋白的可溶性检测···············································184.3融合蛋白的纯化························································194.4融合蛋白的酶切与纯化····································

7、···········204.5融合蛋白与非融合蛋白的活性检测································215分析与讨论··································································22第二部分FGF9亚家族（FGF9/16/20）对HuH7细胞增殖和迁移的影响1引言···········································································242

8、实验仪器和材料····························································243实验方法·····································································253.1HuH7细胞的培养与保存··································