槐杞黄清膏对小鼠肾脏系膜细胞增殖及细胞周期影响的研究

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1、分类号：R5学校代码：10114密级：学号：151531040槐杞黄清膏对小鼠肾脏系膜细胞增殖及细胞周期影响的研究Effectofhuaiqihuangcreamonproliferationandcellcycleofmousekidneymesangialcells研究生：暴宇指导教师：李荣山教授专业名称：内科学研究方向：肾功能不全的基础与临床学位类型：专业学位所在学院：附属人民医院中国山西二〇一八年三月二日目录中文摘要.................................................

2、...............................................................................I英文摘要..............................................................................................................................III常用缩写词中英文对照表.......................................

3、............................................................V前言....................................................................................................................................11材料与方法.............................................................

4、........................................................21.1实验材料..................................................................................................................21.1.1细胞.............................................................................

5、...................................21.1.2主要材料及试剂............................................................................................21.1.3主要仪器........................................................................................................31.2实验方法.

6、.................................................................................................................31.2.1系膜细胞的培养............................................................................................31.2.2实验分组...................................

7、.....................................................................51.2.3CCK-8检测细胞增殖活力............................................................................51.2.4流式细胞仪检测细胞周期分布....................................................................51.2.5Wester

8、nBlotting检测CyclinA及p21的表达............................................61.2.6统计学方法...............................................................................