il4il10诱导的树突状细胞对类风湿性关节炎的作用

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1、·１１０４·中国免疫学杂志２０１１年第２７卷doi：１０．３９６９／j．issn．１０００-４８４X．２０１１．１２．１２①IL-4／IL-10诱导的树突状细胞对类风湿性关节炎的作用②③聂瑛洁周晓泉袁军安宇肖林生（贵州省人民医院中心实验室，贵阳５５００００）中国图书分类号R392．12文献标识码A文章编号1000-484X（2011）12-1104-06［摘要］目的：探讨经IL-４／IL-１０诱导的树突状细胞（DC）对类风湿性关节炎的作用。方法：用Percoll分层离心法从脾脏细胞分离得到DC后，

2、用IL-４或IL-１０或IL-４＋IL-１０进行诱导。用未经诱导和诱导过的DC对类风湿性关节炎大鼠模型进行干扰。SD鼠设为CIA模型组，DC对照组与DC试验组。用ELISA法检测细胞因子与抗体水平，用MTT法检测细胞增殖情况，对鼠爪关节行病理学检测。结果：DC对照组的临床症状评分，病理改变评分，细胞增殖能力，血清中抗胶原抗体及细胞因子水平与CIA模型组的差异无统计学意义。试验组中，注射IL-１０-DC能改善CIA鼠的滑膜炎症情况；在初次免疫后第５天注射IL-４-DC能减轻CIA鼠的滑膜炎程度；注射

3、IL-４＋IL-１０-DC无明显的保护作用。结论：适时注射IL-４或IL-１０诱导的DC，对实验性类风湿性关节炎具有保护作用。［关键词］树突状细胞；自身免疫性疾病；类风湿性关节炎；白细胞介素ThepotentialofIL-4／IL-10pre-treateddendriticcellsonrheumatoidarthritisNIEYingJie，ZHOUXiaoQuan，YUAN-Jun，ANYu，XIA-OLinSheng．TheClinicalLaboratory-Centre，Guizh

4、ouProvincialPeople’sHospital，Guiyang５５００００，China［Abstract］Objective：ToinvestigatethepotentialtherapeuticfunctionofIL-４／IL-１０pre-treateddendriticcells（DC）intreatingrheumatoidarthritis．Methods：DCswereisolatedfromtotalspleencellsbycentrifugationat３０００r／m

5、infor３０mininPercell．ThentheyweretreatedwithIL-４／IL-１０／IL-４＋IL-１０．SDratsweredividedintothreegroups，DCcontrol，Collageninducedarthritis（CIA）modelandDCex-perimentalgroup．DCspre-treatedwithdifferentcytokineswereinjectedintravenouslytotreatSDrats．Thelevelof

6、cytokinesandanti-BⅡCweretestedwithELISA；thecellproliferationwastestedwithMTT；thepathologicanalysisoftherawwasperformed．Results：Abouttheclinicalscore，thescoreofpathologicanalysis，theproliferation，thelevelofanti-BⅡCandtheproductionofrelatedcytokineswere

7、tested．TherewerenostatisticaldifferencesbetweenDCcontrolandCIAmodel．SDratsbenefitedfromIL-１０-DCtreatment，butIL-４-DConlyhadtheprotectivefunctionwhentheywereinjected５daysafterthefirstimmunization．IL-４＋IL-１０-DCdidnotexhibitprotectivefunctionintreatingSDr

8、ats．Conclusion：IL-４-DCandIL-１０-DCinjectedatsuitabletimepointofthediseaseprocesshavethepotentialintreatingRA，butIL-４＋IL-１０-DCmayhavenosuchfunction．［Keywords］Dendriticcells（DC）；Autoimmunediseases；Rheumatoidarthritis（RA）；Interleukin（IL）树突状细胞（Dend