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ID:32354683
大小:8.96 MB
页数:44页
时间:2019-02-03
《il17a对gmcsf诱导的骨髓树突状细胞的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、天津医科大学硕士学位论文costimulatorymoleculesCD40,CD86andMHCIIbyinductionGM.CSEAtthesametime.ithasadose.dependent.InordertoconfirmtheeffectofIL.17AtomllydifferentiatedBMDC,weaddedrmlL.17(50,100or500ng/m1)toBMDCinducedbyGM.CSFfor8days.thencontinueculture36hours.Compared、析tIlN0rmlL.17
2、A,BMDCsurfacecostimulatorymoleculesCD40,CD80,CD86,MHCIIandthecytokinessecretioninthesupernatantIL.12p40andIL.10werenotsignificantlychangedofeachgroup.ItshowsthatIL一17AhasnofunctionofstimulatingfullydifferentiatedBMDCtomature.ButIL.17Aup.regulatestheexpressionofcostimulato
3、rymoleculesonDCandthesecretionofIL.12P40andIL.1OintheprocessofGM.CSF.induced.BMDCwithLPS.Conclusions:T11iSexperimentusesthemethodofGM.CSF.inducingandLPSstimulizationsuccessfullyculturedandamplificationDC.IL.17ApromotesthedevelopmentofDCprogenitorsphenotypewhichisinducedby
4、GM-CSF.IL一17AalonehasliRleinfluenceonthedifferentiationofDC.IL.17Aup.regulatestheexpressionofDCcostimulatorymoleculesincludingCD40.CD80.CD86andMHC11whichaleexpressedonmatureDCstimulatedbyLPS.Italsoup—regulatethelevelofIL一12p40andIL一10secretedbyDCinducedbyLPS.Thesefindings
5、suggestthatIL一17AplaysanimportantroleinpromotingDCdifferentiation.Keywords:BMDCIL一17AGM—CSFDCcostimulatorymoleculeIL··12p40IL·-10IV天津医科大学硕士学位论文目录中文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯IAbstract·······················································································
6、II符号说明⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯VI前言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·1月IJ舌⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。l研究现状、成果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯2研究目的、方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯3对象和方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯41实验材料⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯41.1主要试剂⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯4
7、1.2主要仪器和设备⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯51.3主要试剂的配制⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯51.4实验动物⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯72实验方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯72.1小鼠BMDC的分离和培养⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯72.2CDllc免疫磁珠分离提纯成熟BMDC⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯82.3流式细胞术检测成熟BMDC表面分子⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯82.4ELISA检测细胞因子分泌⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯
8、⋯92.5统计分析⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯10结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯11l小鼠骨髓来源的DC体外扩增方法的建立⋯⋯⋯⋯⋯⋯⋯
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