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1、·664·中国实验血液学杂志JournalofExperimentalHematology2005;13(4):664-668文章编号(ArticleID):1009-2137(2005)04-0664-05·论著·+抗TGF2β抗体对人脐血CD34细胞体外扩增及黏附分子表达的影响钱筠,刘复强,吴轶苹,王阳,康文婷首都医科大学附属北京同仁医院血液科,北京100730++摘要本研究的目的是证实抗TGF2β抗体在人脐血CD34细胞体外扩增过程中可以增加CD34细胞的扩增效++率,并观察其对脐血CD34细胞粘附分子
2、表达的影响。从6份新鲜脐血标本中富集CD34细胞,将其接种于含抗TGF2β抗体+SCF+Flt23L+TPO+IL23因子组合的SFEM无血清培养体系中,培养6天后,细胞计数,并用FACS检测CD34、c2kit(CD117)、CD11a、CD49d、CD33表达情况,同时进行集落分析。结果表明:①实验组有核细胞数、+++CD34细胞数、CD34c2kit细胞数及CFU2GEMM分别扩增了41.82±13.49,15.62±6.95,13.36±6.12,11.07+-±4.05倍,明显高于对照组(P分别=0
3、.001,0.002,0.003,0.002),其中实验组中更为早期的CD34c2kit亚群的+++扩增倍数更多(69.10±41.06),多于有核细胞、CD34细胞、CD34c2kit细胞的扩增倍数(P=0.024)。②TGF2β抗体对CD11a和CD49d的表达无明显影响(P值均大于0.05)。结论:抗TGF2β抗体可协同早期干细胞生长因子++有效扩增脐血CD34细胞,同时不降低CD34细胞黏附分子的表达。+关键词抗TGF2β抗体;脐血;体外扩增;CD34细胞;粘附分子中图分类号R329.28;Q26;R
4、331.1文献标识码AEffectofAnti2TGF2βAntibodyonexvivoExpansionandExpres2+sionofAdhesionMoleculesofHumanCordBloodCD34CellsQIANJun,LIUFu2Qiang,WUYi2Ping,WangYang,KangWen2TingDepartmentofHematology,BeijingTongrenHospitalAffliatedtoCapitalUniversityofMedicalSciences,Be
5、ijing100730,ChinaAbstractTGF2β,asaninhibitorofhemopoiesis,excretedbyhematopoieticstemandprogenitorcells,down2regu2latestheexpressionofcytokinessuchasFlt23ligand,SCF,IL23etconthestemandprogenitorcells.Theeffectofanti2+TGF2βantibodyonexvivoexpansionandexpress
6、ionofadhesivemoleculesoncordbloodCD34cellswasstudiedin+thisresearch.TheCD34cellsfromsixunitsoffreshumbilicalcordbloodwereenrichedbydensitygradientsedimenta2tionandpurifiedbyminiMACScellisolationsystem,andplatedthemintotheSFEMserumfreeculturesystemwhichconta
7、iningSCF,Flt23L,TPOandIL23intheconditionof37℃,5%CO2,andsaturatedmoisture.Therewerethreegroupsinthisexperiment:(1)blankgroup:sameastheculturesystemdescribedabove;(2)controlgroup:addedwithnormalrabbitIgGintothementionedculturesystem;(3)testgroup:thesamecultur
8、esystemwithanti2TGF2β1antibo2dy.Culturedfor6days,thenumberofmononuclearcells(MNC)wascounted,theexpressionofCD34antigen,CD117(c2kit)antigen,CD11aantigen,CD49dantigenandCD33antigenwastestedwithFCM.Meanwh
