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页数:50页
时间:2019-03-06
《褪黑素增强顺铂对人食管癌细胞的增殖抑制作用及其可能机制》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、安徽医科大学硕士/博士学位论文分类号:密级:UDC:编号:学位论文褪黑素增强顺铂对人食管癌细胞的增殖抑制作用及其可能机制SynergisticallyAntiproliferativeEffectofMelatoninandCisplatinonHumanEsopHagealCancerCellLinesandthePossibleMechanism刘玲玲指导教师姓名孙国平教授安徽医科大学(至院、所)申请学位级别硕士专业名称肿瘤学提交论文日期2013-04论文答辩日期2013-05学位授予单位和日期安徽医科大学答辩委员会主席评阅人2013年05月1安徽医科大学硕士/博士学位论文
2、安徽医科大学ANHUIMEDICALUNIVERSITY硕士学位论文论文题目褪黑素增强顺铂对人食管癌细胞的增殖抑制作用及其可能机制SynergisticallyAntiproliferativeEffectofMelatoninandCisplatinonHumanEsopHagealCancerCellLinesandthePossibleMechanism作者姓名刘玲玲指导老师孙国平教授学科专业肿瘤学研究方向肿瘤个体化治疗论文工作时间2011年12月至2013年4月2安徽医科大学硕士/博士学位论文学位论文独创性声明本人所呈交的论文是我个人在导师指导下进行的研究工作及取得的研
3、究成果。据我所知,除了文中特别加以标注和致谢的地方外,论文中不包含其他人已经发表或撰写过的研究成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确说明并表示谢意。学位论文作者签名:日期:学位论文使用授权声明本人完全了解安徽医科大学有关保留、使用学位论文的规定:学校有权保留学位论文并向国家主管部门或其指定机构送交论文的电子版和纸质版,有权允许论文进入学校图书馆被查阅,有权将学位论文的内容编入有关数据库进行检索,有权将学位论文的标题和摘要汇编出版。愿意将本人的学位论文提交《中国博士学位论文全文数据库》、《中国优秀硕士学位论文全文数据库》和《中国学位论文全文数据库》中全
4、文发表,并可以以电子、网络及其他数字媒体形式公开出版,并同意编入CNKI《中国知识资源总库》,在《中国博硕士学位论文评价数据库》中使用和在互联网上传播。保密的学位论文在解密后适用本规定。学位论文作者签名:导师签名:日期:日期:3安徽医科大学硕士/博士学位论文目录中文摘要···················································7英文摘要···················································81.前言·············································
5、········102.实验材料·················································82.1细胞株·················································122.2药物及试剂·············································122.3仪器与设备·············································133.实验方法················································143.1
6、细胞的培养与传代·······································143.1.1细胞的冻存和复苏·····································143.1.2细胞换液·············································153.1.3细胞传代·············································163.2细胞活力的测定········································163.3体外增殖抑制实验················
7、······················163.4药物相互作用指数评价药物相互作用······················173.5集落克隆染色·········································173.6流式细胞术检测细胞凋亡································183.7WesternBlot检测Bcl-2、Bax蛋白表达····················123.7.1食管癌细胞Eca109总蛋白的提取········
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