桦褐孔菌野生菌丝体和培养菌丝体的甾体类化合物组成

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1、维普资讯http://www.cqvip.com·750·药学学报ActaPharmaceuticaSinica2007，42(7)：750—756Sterolcompositioninfield-grownandculturedmyceliaofInonotusobliquusZHENGWei—faH，LIUTong，XIANGXiao—yan，GUQiKeyLaboratoryforBiotechnologyonMedwinalPlantsofJangsuProvince，XuzhouNormalUniversity，Xuzhou221j16，China；2．Schoo

2、lofLifeSciences，AnqingNormalCollege，Anqing264000，China)Abstract：Sterolsareoneo±theactiveclasseso±compoundsinInonotusobliquusfortheireffectivetherapyofmanydiseases．Infieldenvironment，thisfungusaccumulateslargeamountofsterols．Inculturedmycelia，however，thisclassofcompoundsislessaccumulated．Fo

3、ranalyzingthefactorsresponsiblefordifferingsterolcomposition，thefield·grownandculturedmyceliawereextractedwith80％ethanolatroomtemperatureandtotalsterolswerepreparedusingsilicongelcolumnchromatographyfollowedbyidentificationusingeitherGC—MSorspectroscopicmethods．ForculturingInonotusobllquus

4、，theseedculturewasgrowneitherinbasicmediumconsistingofglucose(2％)，yeastextract(0．5％)，KH2PO4(0．01％)，MgSO4·7H2O(0．05％)anddistilledwateratpH6．5，orthebasicmediumsupplementedwithserialconcentrationsofAgNO3．Theresultsindicatedthatfield—grownmyceliacontainedlaflosterolandinotodiol(comprised45．47％

5、and25．36％ofthetotalsterols。respectively)andother10sterols(comprisingtheremaining30．17％)includingergosterolbiosyntheticintermediatessuchas24·methylenedihydrolanosterol，4，4-dimethylfecosterol，4-methylfecosterol，fecosterolandepistero1．Columnchromatographyalsoledtotheisolationoflanosterol，Inot

6、odiol，trametenolicacid，foscoparianolBandanewtriterpenoidfoscoparianolDinfield-grownmycelia．Incomparison，theculturedmyceliaonlycontainedthreesterolswithergosterolasthepredominantone(82．20％)．Lanosterolonlyaccountedfor3．68％．SupplementingAgintothecuhureat0．28Ixmol·Lgreatlyenhancedcontentoflano

7、sterol(accountingfor56．81％)anddecreasedthecontentofergosterol(18．5％)togetherwiththepresenceofintermediatesforergosterolbiosynthesis．Theseresultssuggestedthatthesterolcompositioninmyceliaofthefunguscanbediversifiedbysupplementingsubstancesinhibitingenzymaticpro