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1、#160#中华高血压杂志2010年2月第18卷第2期ChinJHypertens,February2010,Vol.18No.2#论著#阿托伐他汀对压力负荷引起高血压大鼠心肌肌浆网钙泵活力的影响何世保,王安才,陈月云,汪俊元,汪念东=摘要>目的观察阿托伐他汀对压力负荷增高所致左心室肥厚(LVH)和心肌肌浆网钙泵(SERCA)活力的影响。方法雄性SD大鼠50只随机分为假手术组、腹主动脉缩窄(AAC)组、阿托伐他汀10mg/(kg#d)(Ato10mg)、阿托伐他汀30mg/(kg#d)(Ato30mg)和氨氯地平5mg/(kg#d)组,每组10只。采用
2、腹主动脉缩窄术建立LVH动物模型,灌胃法给药4周。鼠尾容积法测量收缩压,计算左心室质量指数(LVMI),即左心室质量/体质量(LVM/BM);HE染色检测心肌细胞平均直径;无极磷酸根法测定SERCA活力;双波长荧光分光光度计检测心肌细胞内Ca2+浓度。结果与假手术组相比,AAC组收缩压、LVMI、心肌细胞平均直径、细胞内Ca2+浓度均显著升高,心肌SERCA活力(410?016)比假手术组(614?018)Lmol/(mgpro#h)明显降低(均P<0105)。与AAC组比较,阿托伐他汀30mg组及氨氯地平组均能降低大鼠收缩压、LVMI、心肌细胞平均
3、直径和细胞内Ca2+浓度,同时能明显提高心肌SERCA活力[Ato30mg组(419?014),氨氯地平组(418?015)比AAC组(410?016)Lmol/(mgpro#h),均P<0105]。结论阿托伐他汀改善腹主动脉缩窄高血压大鼠LVH可能与增加SERCA活力,降低压力负荷诱导的肥厚心肌细胞中Ca2+浓度有关。=关键词>阿托伐他汀;高血压;左心室肥厚;肌浆网钙泵;大鼠EffectofAtorvastatinonActivityofMyocardialSarcoplasmicReticulumCalciumPumpinPressure-loa
4、dedHypertensiveRatsHEShi-bao,WANGAn-cai,CHENYue-yun,WANGJun-yuan,WANGNian-dong.DepartmentofGeri-atrics,AffiliatedYiJiShanHospital,WannanMedicalCollege,WuhuAnhui241000,China=Abstract>ObjectiveToinvestigatetheeffectofatorvastatinonleftventricularhypertrophyinducedbypres-sureoverl
5、oadandtheactivityofmyocardialsarcoplasmicreticulumcalcium(SERCA).MethodsLeftventricularhypertrophywasinducedbyabdominalaorticconstriction(AAC)inadultSDrats,andreceivedatorvastatin10mg/(kg#d)(n=10),atorvastatin30mg/(kg#d)(n=10),amlodipinegroup(5mg/kg#d)(n=10).Shamoperatedrats(n=
6、10)anduntreatedAACrats(n=10)servedascontrols.Systolicbloodpressure(SBP)andLV-MIweredetermined,cardiomyocytesdiameterweremeasuredbyHEstain;theactivityofmyocardialSERCAwas2+determinedbynon-polarphosphatemethod;Caconcentrationincardiomyocyteswasdetectedbydualwavespec-trofluoromete
7、r.ResultsComparedwithshamoperationgroup,SBP,LVMI,meandiameterofcardiomyocytesandCa2+concentrationofcardiomyocytesinAACratsweresignificantlyincreased;whiletheactivityofmyocardiumSERCA(410?016)vs(614?018)Lmol/(mgpro#h)wassignificantlydecreased(allP<0105).SBP,LVMI,meandiameterofca
8、rdiomyocytesandCa2+concentrationofcardiomyocytesinAACr
