共生转录因子表达载体的构建与转基因水稻培育

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1、—————————————兰皇壅些塑!!堡堡主型壅竺兰垡!望些!笙塞AbstractAMsymbiosesandrootnodulesymbiosisaretwospecialsymbioticfonll．Withthestudyofthesymbiosismechanism，peoplegetsomeconsensusontheevolutionarvrelationshipsbetweenthem．RootnodulesymbiosisistobeevolvedfromAMsymbiosis，Geneticlocioftheplan

2、tgenomebetweenrootnodulesymbiosisandAMs．vmbioSissignalingpathwaysignalingpathwayexistoverlap．Forexample，partofthericegerlesregulateAMsymbiosisfunctionedinrootnodulessymbiosisoflegUmes，tIlereareatIeastnvericegenesthatregulateAMsymbiosisplayaroleonbothkindofsymbiosis．Thisb

3、ecameanmaportantbasisforexplorationthattransfertherootNodulesymbiosissystemtonon-rootnodulesymbiosisplant．Inrecentyears，theresearchershadclonedaseriesofkeysymbiosisgenesbyllsingthedifferentmutantsofsymbioticdef．eCtsofmodelleg啪e(Lotusjaponciusand：：Medicagotruncatula)．Base

4、dOntheresules，peopleestablishedthemodelofnodulationsignalingtransductionpathway．wetryto缸lansf．erthesekeysymbioticgenesintoriceoestablishanodulationsignalingtransduction．Themainresultsaleasfollows：1·Theconstructionofthecombinatorialexpressionvector：pCAMBIA1302-35sNSPl-35s

5、NSP2—35sN／NandpCAMBIAl302．35sNSPl．35sNSP2—35sN／N．35sLHKl．2·TransformedthepCAMBIAl302，pCAMBIAl302—35sNSPI．35sNSP2．pCAMBIAl302-35sNSPl—35sNSP2—35sN／NandpCAMBIAl302．35sNSPl．35sNSP2-35sNIN-35sLHKlintoNipponbarethroughcallus，andgetalotoftransgenicpedigree．3·Tendaysafterinocul

6、ation，bothtransgeneticgroupandcontroIcanobsen厂erhizobiurninvasionoftheepidermisandroothairsandformstmcturessimilartoinfectionthreads。Almostallofwhichhaveappearedbesidet11elate蹦root．4·Rhizobiuminoculationchangedtheapicalportionofthelateralrootdevelopmentofrice-Thenumberof

7、lateralrootprimordiasandlateralrootincressedsignificantlva盘errhizobiurninoculationthantheuninoculatedricerespectivelybothtransgenicriceandcon仃01．Afterinoculatedwithrhizobium，thelateralrootsnumberoftransgenicgroup懈significantlygreaterthanthecontrolgroup．5·Theexpressionofs

8、omesymbioticgeneorthologsoftransgenicgrouphavenobigdItli：rencetransgenicgroupafterinoculatedwithRhizobi