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ID:33956584
大小:2.09 MB
页数:86页
时间:2019-03-02
《结核分枝杆菌重组bb-esat-6-mpt64疫苗的构建及其免疫机制地地研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、重庆医科大学硕士研究生学位论文英文缩写ADCCAmpAPCA:rPBbBCGBSAbpCDcDNAConACTLCFUDaDABDEPCDMSODNascDW西mELISAEBEDTAEp符号说明英文全称Antibody—dependentcellularcytotoxicityAmpicillinAntigen—presemingcellAdenosinetriphosphateBifidobacteriumbifidumBacillusCalmetteGuerinBovinescramalbuminbasepairCluslerofdiffer
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3、ndoff1中文全称抗体依赖性细胞介导细胞毒性氨苄青霉素抗原提呈细胞三磷酸腺苷两歧双歧杆菌卡介苗牛血清白蛋白碱基对分化群互补脱氧核糖核酸刀豆蛋白A细胞毒性T细胞克隆形成单位道尔顿二氨基联苯胺焦碳酸二乙酯二甲亚砜DNA酶蒸溜水三磷酸脱氧核糖核苷酸酶联免疫吸附试验溴化乙锭乙二胺四乙酸微量离心管重庆医科大学硬士研究生学位论文ES』^T-6FCMHIvHRPHSPⅢN.YIg【LPTGmRNAMTTNC衄6kDaearlysecretoryantigenic6KDa早期分泌性抗原靶targetflowcytometerhumanimmunodeficien
4、cyvirushorseradishperoxidaseheatshockproteininterferon-YImmunoglobulinintefleukinisopropylthio-13-D·galaetosidekilo—basepairblo.DaltonLuria-Bertani(medium)MultipleclonesitesMajorhistoeompatibilitycomplexMycobacteriumtuberculosism,crogrammicroliterMicrometerMinuteMilliliterM.mo
5、I/LMessageribonucleicacid3一(4,5一dimcthylthiazo-2-y1)2,5-dip—henyltetrazoliumbromideNitrocellulOSeNanometer2流式细胞仪人类免疫缺陷病毒辣根过氧化物酶热休克蛋白Y干扰素免疫球蛋白白细胞介素异丙基硫代-18.D.半乳糖苷千碱基对千道尔顿LB培养基多克隆位点主要组织相容性复合物结核分枝杆菌微克微升微米分钟毫升毫摩尔/升,千分之一克分子浓度信使核糖核酸四甲基偶氮唑盐硝酸纤维素纳米妨眈u懈一mM¨岫.昌皿删重庆医科大学硕士研究生学位论文NSODORFR~
6、GEPBMCPBSPCRPgpmolPHArBb.ESAr.6-MPT64r/minCrpm)RNArRNARNaseSDSsecStTAETaqTBTCRTEⅣ匝D弧TNF.aTris嘣s-Clnormalsaline(O.9%NaCl)optiealdensityOpelReadingFramePolyacrylamidegelelectrophoresisPeripheralbloodmononuclearcellphosphate—bufferedsalinepolymerasechainreactionPictogramPieomolPh
7、ytohemagglutininrecombinantBb.ES』蛆巧一~口T64vaccinerotationperminuteribonueleicacidRibosomalRNARibonucleasedodecylsodiumsulfateSecondSalmonellatypbamuriamTfis/acetateelectrophoresisbufferThermus—aquatic懈DNApolymeraseTuberculosisTcellantigenreceptorN,N,N’N'-tetramethylethylenediam
8、ineHelperTcellTumornecrosisfactor-aTris—hydroxymethylaminomet
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