姜黄素对大鼠脑缺血后神经细胞增殖的影响

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1、温州医学院硕士学位论文3．姜黄素腹腔注射给药(300mg／kg)上调MCAO后NICD的表达，这可能是姜黄素能促进神经细胞增殖并最终达到神经保护作用机制所在；【关键词】脑卒中；姜黄素；神经保护；神经细胞增殖；Notch温州医学院硕士学位论文NeurogenesisEffectafterIschemiconRatfromCurcuminAbstract[Propose】Objectingtoinvestigateneurogenesiseffectonfocalischemiareperfusioninjuryandregulatemechanismofnotchproteininnotchsi

2、gnalpathfromcurcumin。[Method】ThefocalisehemiareperfusioninjurymodelwasinducedbymiddlecerebralarteryocclusionandthereWasthreegroupsdivided：sham—operatedgroup(Shamgroup，n=15)，vehiclegroup(Vehiclegroup，n=15)andcurcumingroup(Curcumingroup，n=15)．PulloutsutureaRer2handfinishedthefocalisehemiarepcrfusion岫瑚

3、了model．modclCurcumingroupWastreatedwithcurcumin(300mg／kg)atlhafterstrokelastingfor7daysbyintraperitonealinjectionandtheothergroupswereinjectedwithequalvolumecoiloil7dtoo，12dkilledafterMCAO．NeurobehavioraldeficitofZealongaWasappliedtodividegroupafter22hfromreperfusionandneurobehavioraldeficitWasuseda

4、fterId，3d，7daswellas12d．BrdU，BrdU／DCXexpressionaswellasNICDrelativelevelwerestudiedinourexperimentbyimmunohistochemieal，immunofluorescenceandwesternblottingtechnique．[Results]1．mNSS：Ascompared、Ⅳimvehiclegroup，curcumingroup’Sncurobehavioraldeficithasadescendtrendat3d，7d，12d．ThereWasastatisticaldiffer

5、encebetweencurcumingroupandvehiclegroupwimBrdUpositivecells(木P<0．05)．ThereWasnostatisticaldifferencebetweencurcumingroupandvehiclegroup、)IrimBrdUpositivecellsat1d俨>O．05)2．BrdUExpression：BrdUexpres,：；ion(brown)inSVZtestbyImmunohistochemicalstaining．BrdUexpressioncellscountamongshamgroup，vehiclegroupa

6、ndcurcumin．Comparedtoshamgroup，vehiclegrouppositivecellshasstatisticalmeaning(木P

7、legrouppositivecellshasstatisticalmeaning(木P<使05)．Comparedtovehiclegroup，curcumingrouphasastatisticalmeaning(幸宰P<0．01)．4．NICDproteinexpression：NICDproteinexpressionincurcumingrouprats4．．望型垦兰堕婴主兰篁垒茎一一—