急性缺血脑组织靶向肽的筛选、鉴定与分子成像分析

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2、itioningofthesestressresponseareconduciVetoindiVidualtherapyandpredictpro罂losis．MoleculafImagingenablestheVisualisationandcharacterizalionofthecellular‰ctioninliVingorganismsbySpecialmolecularprobes，whichavailableformoIlitoriIlgpa也ologicalproceSsaftercerebra

3、lischemia．HoweVer，mereweref色wreportsonmespecificmolecularprobesforst】．okemolecularimaging，limitingt11eapplicalionofm01ecularimagingtechnology．Therefore，itisa11u唱entneedtoseekmolecularprobesforstrokemolecularimaging．Inthepresentstudy’纫1，如Dphagedisplaytecllnol

4、ogywaSusedtoidenti匆peptides廿latspecificallybindtocerebralischemiatissue，aIldmolecularprobewasbuildedbasedonspecialpeptide．7I’hisdissertationincludedthefollo、ⅣingthreepaJts：1．砌vfvDscreeningofpeptidestllatSpecificallybindtocerebralischemia虹ssue．TheC57tmsientri

5、ghtmiddlecerebraJarteryocclusion(MCAO)modelswereestablishedbysuturemethod，and砌v如DscreeIlingwaSc邺redoutbyPh．D．．7TMphagedisplaypeptidelibrary．AnerfourroundsofscreeIling，therecoVeredphagesatipsilateral(ischemic)hemisphefeweree埘chedapproximatelybyl0．4一foldascomp

6、aredtothefirstround．120phageclonesf．romthefounhround、vererarldomlypicked，thenthepeptide—codingDNAinsertsweresequencedandtranslatedintocorrespondingpeptidesequences，and17peptideswereacquiredatlaSt．Themost仃equentphageclonediSplayingHGGVR【Ypeptide(namedHGGasthe

7、phagenamedHGG—M13)waSselectedforfmherexperiments．2．IdentificationofHGG—M13phageandHGGpeptidehomingtothes仃oketissue．Thespecialit)rofHGG—Ml3homingtothes仃oketissuewaScharacterizedbytiteringofphageandimmunonuorescenceinHGG-M13intmVenouslyadmillisteredtoMCA0mice，

8、andthespecialitywasgiRbyHGGpeptidedisplayedonphage，、vhilenaItthephage．Further’HGG—Ml3boundtoneuronsbutnottoaS仃oc”esatmeischemicstmketissuebydoubleIIIAbstractimmunofluorescentstajIlingexperimems．