t细胞来源exosomes调节妊娠丢失孕鼠母胎界面ctla-4foxp3表达的分析

《t细胞来源exosomes调节妊娠丢失孕鼠母胎界面ctla-4foxp3表达的分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、万方数据英文摘要StudyoftheexpressionofCTLA．4andFoxP3frommicewithpregnancylossinducedbyadoptivelytransferringExosomesfromTlymphocytesABS’瞰CTObjective：ThenormalpregnancydependsonmaternalimmunetolerancetotheembryoandhalfthesalD．eantigen，oncethestatehasbeenbrokenwouldlea

2、dtotheoccurrenceofabortion．Continuous3timesormorethen3timesrepetitivepregnancylossofpatients，whoabout50％，thereisnoclearclinicaletiology,calledunexplainedrecurrentspontaneousabortion(URSA)．Studyofmaternal—fetalimmunetoleranceintheprocessofpregnancytheexactmech

3、anisms，inducingtheformationofmaternal_fetalimmunetoleranceisthekeytotreatmentofURSA．Methods：1PreparationofsplenicmononuclearcellsandExosomesfromunrelatedindividuals：BALB／cmalemiceweresacrificed，andthesuspensionofsplenocyteswascollected．ExosomesfromTlymphocyte

4、swereisolatedbysucrosegradientultracentrifngationandultrafiltration．2Establishmentofanimalmodelandexperimentalgroups：Thefemaleandmalemicewererandomlydividedintogroupswithmating2：1，CBA／J(早)xBALB／c(艿)ascontrolgroupofnormalpregnancy(Contr01)andCBA／J(早)xDBA／2(8)a

5、sURSAexperimentalanimalmodel．TheCBA／JmicefromURSAgroupwererandomlydividedintoURSAgroup(Onthefourthdayafterpregnancy，wereinjectedintravenouslywithsodiumchloride)，CellularTherapygroup(Onthefourthdayafterpregnancy，wereinjectedintravenouslywithsplenocytesfromunre

6、latedBALB／cmalemice)，Non．cellularTherapygroup(Onthefourthdayafterpregnancy，wereinjectedintravenouslywithExosomesderivedfromsplenocytes)．．3Observationofembryodevelopment：TheCBAJJpregnantmiceineachgroupsweresacrificedon14thdays，thevolumesofplacentaweremeasured，

7、万方数据荚父捅要thenumberofabsorbedplacentaandthesurvivalplacentawerecounted，ratesoffetalabsorptionandpregnancylosswerecalculated．4AnalysisoftheexpressionofCTLA-4andFoxP3onmaternal—fetalinterface：TheCBA／Jpregnantmiceineachgroupsweresacrificedin14thdaysofpregnancy，and

8、theplacentatissuewerepreparedforparaffinimbeddingslice．Afterimmunohistochemicalstaining，thegrayscale(GS)weremeasuredunderimagineanalysissystemtoshowtheexpressxonofCTLA．4andFoxP3，andtherei