血清蛋白质指纹图谱诊断隐匿性绒毛膜羊膜炎的应用研究

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1、目录中英文缩略词对照表........................................1血清蛋白质指纹图谱诊断隐匿性绒毛膜羊膜炎的应用研究.........2中文摘要..................................................2英文摘要..................................................4背景......................................................7第一部分弱阳离子芯片的选择............

2、..................121引言....................................................122材料....................................................123方法....................................................134结果....................................................165结论............................

3、........................17第二部分应用SELDI-TOF-MS技术筛选隐匿性绒毛膜羊膜炎血清特异蛋白并建立诊断模型.......................................181引言....................................................182材料....................................................193方法...................................................

4、.204结果....................................................215讨论....................................................29全文结论.................................................31参考文献.................................................32附录..................................................

5、...38致谢.....................................................39文献综述及参考文献.......................................40中英文缩略词对照表英文英文全称中文全称HCAHistologicChorioamnionitis隐匿性绒毛膜羊膜炎IAIintra-amnioticinfection宫内感染PPROMpretermprematureruptureof未足月胎膜早破membranesSELDI-TOSurfacedenhancedlaserdesor

6、ption/表面加强的激光解吸F-MSionizationtimeofflightmass电离化飞行时间质谱pectroscopyMALDI-TMatrixassistedlaserdesorption/基质辅助激光解吸附OF-MSionizationtimeofflightmass电离飞时间质谱仪CHAPS3-[（3-cholamidopropyl）3-[3-胆酰胺基丙基-二dimethylammonio]-1-propanesulfonate甲氨基]丙磺酸盐EAMEnergyabsorbingmolecule能量吸收分子WCXWeakcationi

7、cexchanger弱阳离子SPASinapinicacid芥子酸DaDalton道尔顿ACNAcetonitrile乙腈TFATrifluoroaceticacid三氟乙酸TrisTris(hydroxymethyl)aminomethane三羟甲基氨基甲烷IMACImmobilizedmetalaffinitycapture金属离子鳌合DTTDithiothreitol二硫苏糖醇M/ZMass-to-chargeratio质荷比SAXstronganionicexchanger强阴离子1血清蛋白质指纹图谱诊断隐匿性绒毛膜羊膜炎的应用研究中文摘要第一

8、部分目的：通过比较弱阳离子交换磁珠(MB-WCX)即液体芯片与弱阳离子蛋白质芯片（WCX2）技