红曲霉转化中药葛根固体发酵条件研究

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1、红曲霉转化中药葛根固体发酵条件研究安徽农业科学.JournalofAnhuiAgn.Sci.2010,38(4):1707—1708,1745责任编辑张杨林责任校对卢瑶红曲霉转化中药葛根固体发酵条件研究宋艳秋，陈有为(1•大理学院公共卫牛学院，云南大理67100o;2.云南大学省微生物研究所，云南昆明650091)摘要［目的］研究红曲霉转化中药葛根I古I体发酵的条件，以期提高其转化率•［方法］对菌株YM3207转化葛根干粉的固体发酵培养条件和培养基进行了优化试验，通过茵种活化，种子培养，固体发酵培养方法以及正交试验考察了种子培养基，底物投入量，转化温度，转化时间因素的影响与

2、红曲霉转化条件.［结果］红曲霉菌YM3207菌株在YPD液体培养基中28°C,200r/min下培养3d作为种子培养条件最为合适;采用固体发酵方式，以屮药葛根为底物,10%接种量,28°C培养10d为红曲霉菌最佳转化条件;培养基组成为葛根粉:鲜豆渣:米粉为1:1:1,含水量为50%时，能够获得较高葛根素含量的转化产物•［结论］采用该条件发酵转化葛根能够提高葛根主要有效成分含量•利用微生物转化提高中药药效是可行的.关键词红曲霉;葛根;发酵条件屮图分类号S188文献标识码A文章编号0517—6611(2010)04—01707—02StudyonthesnHdFermentaf

3、ionConditionsforTransformingChineseMedicinePuerariaeRadixwithMonascuspurpureusS0NGYan-qiaetal(CollegeofPublicHealth.DaliUniversity.Dali,Yunnan671000)AbstractlObiectivelTheaimwastoresearchtlleconditiononsolidfermen〜tionconditionsofMonascuspurpureusfortransformingChinesemedicinePuerariaeradi

4、xinordcrtoincreasetheconvcrsionrate.fMethodlrrJlesolidfermentationcultureconditionsandthemediumofstrainYM3207fortransformingthedrypowderofPuerariaeradixweremadefortheoptimizationtest.Throughthestrainactivation.seedculture,solidfermentationculturemethodandorthogonaltest.tlleeffectsofsomefac

5、torssuchastheseedmedium.substrateinputamount.transitiontemperature.transitiontimefactOrsandthetransitionconditionofMonascuspurpureuswereinvestigated.1ResuhlTheseedconditionwhenthestrainofMonascuspurpureusYM3207wascuhuredintheYPDliquidmediumunder28°Cand200r/minfor3dwasoptimum.Theopti.mumtra

6、nsitionconditionofMonascuspurpureusstrainwasasfollows:takingChinesemedicineP11〜rariaeradixasthesubstratewitllinoculationamountoflO%andCulturingforlOdat28oCbyusingthesolidfermentationmethod.WhenthemediumcompositionswasPuerariaeradixpowder,freshbeancakeandriceflourwithratioofl:1:landthewater

7、contentas5O%.thetransitionproductwithhigherPuerariaeradixcontentwasobtained.fConclusionlUsingthisfermentationconditionfortransformingPuerariaeradixcouldincreasethemainlyeffectivecormpositioncontentofPuerariaeradixanditwasfeasibletotransfoI-inandincreaseChinese