核心蛋白聚糖对哮喘小鼠气道重塑影响

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1、ft111111111111111111111111111；Y2338655肺组织TGF．B1阳性表达平均光密度(MOD)值比对照组显著增高【(O．32士0．04)VS(O．11-4-0．01)，P<0．01】；而decorin干预组小鼠MOD值较气道重塑组明显降低『(O．21士0．02)VS(O．32士0．04)，P<0．01]。6．相关性分析：小鼠TGF．pl在肺组织阳性表达MOD值与WAt／Pbm、WAi／Pbm、WAm／Pbm及Wcol／Pbm成正相关(产O．859、0．799、O．854、0．855；P均<0．01)

2、。结论：1．Decorin可减轻哮喘小鼠气道重塑的发生。2．TGF．131参与了哮喘气道重塑过程，decorin有可能是通过抑制TGF一131干预了哮喘气道重塑。硕士研究生杜彩霞(内科学，呼吸)．导师杜建新副主任医师关键词：哮喘；气道重塑；小鼠；转化生长因子．Ill；核心蛋白聚糖EffectsofdecorinonairwayremodelinginasthmaticmiceAbstractobjeetive：Tostudytheeffectsofdecorin0nairwayremodelingandtheexpressio

3、noftransforminggrowthfactor-lal(TGF一81)inasthmaticmice，andtoexplorethevalueofdecorinonasthmatictherapy．Methods：Thirtyfemalekunmingmicewererandomlydividedinto3groupsincludingthesalinecontrolgroup，theasthmaairwayremodelinggroupandthedecorininterventiongroup，with10mice

4、ineachgroup．Themiceintheasthmaairwayremodelinggroupandthedecorininterventiongroupweresensitizedbyintraperitonealinjectionsofovalbumin(OVA)ondays0，7and14．Fromday22，themicewererepeatedlychallengedwithacrosolizedOVA(2．5％)for30mirddaythreetimesweeklyfor8weeks．Themiceint

5、hedecorininterventiongroupwereintraperitoneallyinjectedwithdecorin2hbeforeeachchallenge．Themiceinthesalinecontrolgroupweresensitizedandexposedtosalineasthesameschedule．AllmiceWeresacrificed24hafterthelastOVAexposure．TGF-131inseruillandthebronchoalveolarlavagefluid(B

6、ALF)weremeasuredbyenzyme-linkedimmunosorbentassay(ELISA)．Thepathologicalchangesofthelungtissueswereobservedbyhaematoxylin-eosin(HE)staining．CollagendepositionswereobservedbyMassonTrichromestaining。Theperimeterofbasementmembrane(Pbm)，totalairwaywallthickness(WAt／Pbm)

7、，innerairwaywallthickness(WAi／Pbm)，airwaysmoothmusclethickness(WAm／Pbm)，andthethicknessofairwaycollagendeposition(Wcol／Pbm)weremeasuredusingallimageanalysissystem．TheexpressionsofTGF-131inlungsweredetectedbyimmunohistochemistry．Results：1．EOS％inBALF：theEOS％inBALFofmi

8、ceinasthmaairwayremodelinggroupweresignificantlyhigherthanthatofsalinecontrolgroup(16．60士2．18％VS7．50d：1．45％，P<0．01)；andtheEOS％inBALFofmice