白藜芦醇对胃癌bgc-823细胞中凋亡相关基因pdcd5表达影响地研究

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1、郑州大学2以拍届硕士研究生毕业论文白黎芦醉对胃癌BGC名23细胞中凋亡相关基因PDCDS表达影响的研究leukemia，lungcaneerandbreasteancer，etal.IteansignificantlyinhibitavarietyofhumantumoreellgrowthandinducedaPOPtosis.ResearchsshowedthatresveratroleanPlayaroleincaneerbyanti一oxidation，inhibiteyclooxygenase(CO均andeytochromeP450enzymes，indueedtumoreells

2、differentiationandPromotetumoreellsaPoPtosisand50on.InordertoresearchtheeffectofPDCDSProteinandmRNAexPressioninhumangastricpoordifferentiatedadenocareinomaeellBGC一823indueedbyresveratrolandtheroleofresveratrolonBGC一823cellgrowthinhibition，thisresearehdrawsuPtousetheimmunocytochemistrySpexaminationpD

3、CDSProteinexPressioninBGC一823eells:andusetheinsituhybridzationexaminationPDCDSmRNAexPressioninBGC一823cells:andusefloweytometryandTUNELexaminationProliferationandapoPtosisofBGC一823cells.ResearchthecorrelationbetweenPDCDSexPressioninBGC一823eellsandBGC一823cellsaPoPtosis，toProvideatheoriesbasisforelinie

4、altreatmentofgastrieeaneer.Materialsandmethods1.SamPles:humangastriePoordifferentiatedadenocareinomaeellBGC一823PurehasefromChineseAcademySeienceShanghaicellbiologygraduateschool.Throughrecoveryineubationandcultured，thecellsdividedintodifferentexPerimentgrouPsanduseZ和mo呱、5助mol/L、1卿才mol几、2001通mol/Lres

5、veratrolhandledBGC一823cellsfor24h，48h，72h，resPeetively.AndsetuPblankcontrolgrouPs(mediumcontrolgrouP)，solventeontrolgrouPs(DMSOcontrolgrouP)，eisPlatineontrolgrouPs(知岁ml)，combinationgrouPs(resveratrol2001‘mol/L+cisPlatin和留ml).2.UseimmunocytochemistrySPmethodtodetecttheexPressionofPDCDSProteininBGC一82

6、3eellsofeaehgrouPs.3.UseinsituhybridzationmethodtodetecttheexPressionofPDCDSmRNAinBGC一823cellsofeachgrouPs.4.UseFloweytometrytoanalyzethecellcyeleofeachgrouPsfor24h，48，72h，resPectively.郑州大学2以为届硕士研究生毕业论文白葬芦醇对胃癌BGC一23细胞中凋亡相关基因PDCDS表达影响的研究5.UseFloweytometrytodetecttheeellaPoPtosisofeaehgrouPsfor24h，48，

7、72h，resPectively.6.UseTUNELtodetecttheeellaPoPtosisofeaehgrouPsfor24h.7.Statisticalanalysis:AllthedatawereanalyzedbySPSS11.0statistiealsoftwarepackage，thedatawereexpressedbymean土standarddeviation(又士S)