老年大鼠肝脏解偶联蛋白2表达与衰老关系的分析

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1、ResearchontherelationshipbetweentheexpressionofUCP2intheliversofold--agedratsandageingMasterDegreeCandidate：HuXiangshangSupervisor：ProfessorZouYuanMajor：PhysiologyAbstractObjectives：Uncouplingprotein2(UCP2)，discoveredrecentlytobedistributedinmanifoldorgansinrodent，isonememberoftheUCPfamily

2、whichlieinmitochondrialinnermembrane．Actingasaprotonpump，itmayreducetheproductionofreactiveoxygenspecies(ROS)inmitochondria，andperformtheprotectivefunctionwhencellssufferfromoxidativedamage．ManyresearchresultsprovethatROSisthedecisiveelementofageing．Theageingofthelivingorganismappearswitht

3、hereductionofmitochondrialadenosinetriphosphate(ATP)，theincreaseofROS，thesharpeningofitsdamage，andaseriesofchangesoftheturbulenceofneuroendocrinesystem．Therearemanytheoriesaboutthemechanismofageing，amongwhichtherelationshipbetweenfreeradicalandageingiswidelyattended．RespectingUCP2candepres

4、sthelevelofROS，wesupposethatUCP2mayparticipateintheanti·oxidativedamage，consequentlyperformthefunctionofstavingoffageingbyreducingtheoxidativedamageofmediateROScells．Ontheotherhand，UCP2canreducetheprotongradientsacrossinmitochondriamembrane，SOprotoncanentergrassrootsdirectlyinsteadofpartic

5、ipatinginthesynthesrsofATP,leadingtotheexpenditureofthefreeenergyinelectrochemistrialprotentialenergyrestoredinprotonbyuncouplingoxidativephosphoylation．ThelevelofATPdescends．AStherelationshipsbetweenUCP2andROSandATParestillnotclear，SOaretherelationshipsbetweenUCP2，ROS，ATPandageingrespecti

6、vely，wemakeuseofliversofoldratstobeastheresearchingobjecttoillustratestherelationshipsbetweenUCP2andROSandATPinratsofdifferentageandphysiologicstate，exploretheeffectofUCP2onageingandtrytooffertheoreticalcluesforclinicaltreatmentofthediseaseconcernedwithageing3andstarvingofftheageingoforgan

7、s·Methods：MaleSDratsofdifferentagesweredividedintothreegroups：theinfancygroup(21daysafterbirth，n210)，adultgroup(threemonthsafterbirth，n=10)andold—agedgroup(eighteenmonthsafterbirth，n210)．Theseratswerebredforthreedaysindifferentcagesinroomtempraturenaturaldavni