兔骨膜包裹附着有兔骨髓间充质干细胞的plga(乳酸乙醇共聚物)支架材料修复兔大段尺骨缺损的实验分析

《兔骨膜包裹附着有兔骨髓间充质干细胞的plga(乳酸乙醇共聚物)支架材料修复兔大段尺骨缺损的实验分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、浙江大学硕士学位论文英文摘要ReconstructionofsegmentalbonedefectsintherabbitulnausingperiosteumencapsulatedMSCs-loadedPLGAscaffoldsDepartmentofOrthopedics，SecondAffiliatedHospital，SchoolofMedicineZhejiangUniversityPostgraduateSupercisorBackgroundandObjective：XinZhangRongxinHeAbstractRepairoflargebonedefects

2、remainsachallengeforclinicians．Thepresentstudyinvestigatedtheabilityofmesenchymalstemcell(MSC)-and／orperiosteum—loadedpoly(1actic-CO—glycolicacid)(PLGA)topromotenewboneformationwithinrabbitulnarsegmentalbonedefects．Methods：Rabbitbonemarrow-derivedMSCs(passage3)wereseededontoporousPLGAscaffold

3、s．Fortysegme删bonedefects，each15mminlength，werecreatedintherabbitulna，fromwhichperiosteumwasobtained．BonedefectsweretreatedwitheitherPLGAalone(groupA)，PLGA+MSCs(groupB)，periosteum—wrappedPLGA(groupC)orperiosteum-wrappedPLGA／MSCs(groupD)．At6and12weekspost—surgery，samplesweredetectedbygrossobser

4、vation，radiologicalexamination(X—rayandmicro—CT)andhistologicalanalyses．IV浙江大学硕士学位论文英文摘要Results：GroupD，comprisingbothperiosteumandMSCs，showedbetterbonequality，higherX—rayscoresandagreateramountofbonevolumecomparedwiththeotherthreegroupsateachtimepoint伊<0．05)．Nosignificantdifferencesinradiolog

5、icalscoresandamountofbonevolumewerefoundbetweengroupsBandC俨>O．05)，bothofwhichweresignificantlyhigherthangroupA伊<0．05)．TheseresultsdemonstratethatimplantedMSCscombinedwithperiosteumhaveasynergisticeffectonsegmentalboneregenerationandthatperiosteumplaysacriticalroleintheprocess．Conclusions：1)Pe

6、riosteumandbonemarrowmesenchymalstemcellscollaborativeosteogenesis．2)TheperiosteumCanplaytheroleofInduceddifferentiationofbonemarrowmesenchymalstemcellsinthebody．Keywords：mesenchymalstemcell；periosteum；bonedefect；regenerationV目录致谢⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．I中文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

7、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．II英文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯IV目录正文1．序言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．12．材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．42．1多孔的PLGA支架的制作⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯42．2兔骨髓间充质干细胞(MSCs)的提取和体外培养。⋯⋯⋯⋯⋯⋯⋯⋯⋯．．42．3动物体内植入移植物试验⋯⋯⋯⋯⋯⋯⋯