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ID:33070080
大小:4.25 MB
页数:37页
时间:2019-02-19
《兔骨膜包裹附着有兔骨髓间充质干细胞的plga(乳酸乙醇共聚物)支架材料修复兔大段尺骨缺损的实验分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、浙江大学硕士学位论文英文摘要ReconstructionofsegmentalbonedefectsintherabbitulnausingperiosteumencapsulatedMSCs-loadedPLGAscaffoldsDepartmentofOrthopedics,SecondAffiliatedHospital,SchoolofMedicineZhejiangUniversityPostgraduateSupercisorBackgroundandObjective:XinZhangRongxinHeAbstractRepairoflargebonedefects
2、remainsachallengeforclinicians.Thepresentstudyinvestigatedtheabilityofmesenchymalstemcell(MSC)-and/orperiosteum—loadedpoly(1actic-CO—glycolicacid)(PLGA)topromotenewboneformationwithinrabbitulnarsegmentalbonedefects.Methods:Rabbitbonemarrow-derivedMSCs(passage3)wereseededontoporousPLGAscaffold
3、s.Fortysegme删bonedefects,each15mminlength,werecreatedintherabbitulna,fromwhichperiosteumwasobtained.BonedefectsweretreatedwitheitherPLGAalone(groupA),PLGA+MSCs(groupB),periosteum—wrappedPLGA(groupC)orperiosteum-wrappedPLGA/MSCs(groupD).At6and12weekspost—surgery,samplesweredetectedbygrossobser
4、vation,radiologicalexamination(X—rayandmicro—CT)andhistologicalanalyses.IV浙江大学硕士学位论文英文摘要Results:GroupD,comprisingbothperiosteumandMSCs,showedbetterbonequality,higherX—rayscoresandagreateramountofbonevolumecomparedwiththeotherthreegroupsateachtimepoint伊<0.05).Nosignificantdifferencesinradiolog
5、icalscoresandamountofbonevolumewerefoundbetweengroupsBandC俨>O.05),bothofwhichweresignificantlyhigherthangroupA伊<0.05).TheseresultsdemonstratethatimplantedMSCscombinedwithperiosteumhaveasynergisticeffectonsegmentalboneregenerationandthatperiosteumplaysacriticalroleintheprocess.Conclusions:1)Pe
6、riosteumandbonemarrowmesenchymalstemcellscollaborativeosteogenesis.2)TheperiosteumCanplaytheroleofInduceddifferentiationofbonemarrowmesenchymalstemcellsinthebody.Keywords:mesenchymalstemcell;periosteum;bonedefect;regenerationV目录致谢⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯.I中文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯
7、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯..II英文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯IV目录正文1.序言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯..12.材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯..42.1多孔的PLGA支架的制作⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯42.2兔骨髓间充质干细胞(MSCs)的提取和体外培养。⋯⋯⋯⋯⋯⋯⋯⋯⋯..42.3动物体内植入移植物试验⋯⋯⋯⋯⋯⋯⋯
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