兔骨髓间充质干细胞同种异体修复关节软骨缺损的实验-.研究

《兔骨髓间充质干细胞同种异体修复关节软骨缺损的实验-.研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、c⋯自大学硕士学忆沧立ABSTRACTObjective：Isolatingandculturinghigh·grademesenchymalstemcellsfromRabbitsbonema丌owandthendifferentiateintochondroblastinvitroToevaluatetheeffectofbonemarrowmesenchymdstroroalcells(BM—MSCs)withSodiumhyaluronatehealingfull-thicknesscartilagedefectsofrabbitknee

2、Methods：1．BonemarrowofrabbitswasobtainedfromproximaltibiabysterilesternumpunctureneedleandReformingensitygradientcentrifugationcultivationwasutilizedtocultureBM·MSCsitculturedintheDMEMwi廿llowglucosecontaining15％fetalbovineserum．Analyzethecellsurfacemarkersbyflowcytometryinpas

3、sage32．BM-MSCsfromthesecondpassageweredividedintotwogroups：theinductiongroupwere，addingthechondrogenicmedium；thecontrolgroupwereaddingthecommonmediumThegrowthoftheculturedcellswasobservedAlcianbluestainingwereusedtodetectthesecretionandexpressionofglycosaminoglycaninextracell

4、ularmatrix．3Eighteenrabbitsweredividedintothreegroupsrandomly．thedefectsoftheexperimentalgroupimplantedBM—ofMSCs／sodiumhyaluronatecomplex；thecontrolgroupwereimplantedSodiumhyaluronate；theblankgroupwithoutspecialtreatmentTheanimalswerekilledin6thand12thweeklater．Restoredtissue

5、wasevaluatedusingnakedeyesandTⅡ中南太学硕J：学位论业∞女捕ghistologicalsection，scorewasgivenaccordingtumodifiedPinedagradestandardThetotalscorewasanalyzedwithstatisticsoftwareSPSS13"0．Results：1．Thesecellpopulationsarecomprisedofmorphologicallyhomogenousfibroblast—llkecellsandpresentplasti

6、c—adherentproperty．FlowcytometryanalysisshowsthatthesecellsarehomogenouslypositiveforthemesenchymalmarkersCD29andCD44，butnegativeforthepan—leukocytemarkerCD452Tileinductiongroupcellsaggregationgrowthasflake—shapedorpolygonal．whichwaspositiveforAlcianbluestaining；controlcellss

7、tillshowedthetypicallongfosiformadherentgrowth，Alcianbluestainingwasnegative．3Cartilagedefectofexperimentalgroupwererepairedwithathin-layerhyaline-likecartilage，whichwaspositivefortype11collagenandAlcianbluestainingcontrolgroupandblankgroupwererepairedwithfibroustissues，which

8、wasweaklypositivefortypeIIcollagenandAlcianbluestainingExperimentalg