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1、三峡大学硕士学位论文AbstractObjectiveTocharacterizetheanti-inflammatorypropertiesofpuerarininamiddlecerebralarteryocclusion–reperfusionratmodel,andinvestigatetheunderlyinganti-inflammatorymechanismofpuerarinfromstimulatingcholinergicanti-inflammatorypathway(CAP).
2、Methods160healthyadultSprague-Daewleyratswererandomlydividedinto6groups:Group1:per-administeredphysiologicalsalinefor5days,buttheoriginoftheMCAOwasnotoccluded,Group2:per-administeredphysiologicalsalinefor5daysbeforeintroductionofMCAO,Group3:per-administ
3、eredpuerarin(36mg/kgintravenousinjection)for5daysbeforeintroductionofMCAO,Group4:per-administeredpuerarin(54mg/kgintravenousinjection)for5daysbeforeintroductionofMCAO,Group5:per-administerednicotine(200μg/kgintravenousinjection)for5daysbeforeintroductio
4、nofMCAO,Group6:thesameprocedureasgroup3,andthenratswereadministeredα-BGT(1.0μg/kg).After2hofischemia,allthegroupsweresubjectedtoreperfusion.Theneurologicalstatuswasmeasuredafter48hreperfusion,andratswerethensacrificedrespectivelyforotherpurposesasfollow
5、:cerebralinfarctsizewasmeasuredbyTTCstainingafter12hreperfusion;L-1β、IL-6、TNF-αandIL-10levelsweredeterminedbyELISA;theexpressionofp-JAK2andp-STAT3weremeasuredbyimmunohistochemistryassay;RT-PCRanalysiswasusedtodecidedtheexpressionofα7nAChR、NF-кBp65、STAT3
6、andJAK2mRNAinbrain;NF-кBp65wasexaminedbyWesternBlottingafter12hreperfusion.Results(1)Comparedwiththecontrolgroup,theneurologicaldeficitscoresweresignificantlydecreasedinthepueraringroupandthenicotinegroup(P<0.05).Thescoreinhighdoseofpueraringroupwaslowe
7、rthaninα-BGTgroup,buttherewasnosignificant(P>0.05).(2)Intheanalysisoflow-dosegroupandnicotinegroupcomparedwithcontrolgroup,significantlydecreasedininfarctvolumewasdetected(P<0.05orP<0.01).Thevolumeofcerebralinfarctioninlow-dosegroupwassignificantlysmall
8、erthanα-BGTgroup（P<0.01).Therewerenosignificantdifferencesininfarctvolumebetweenhighdoseofpuerarinandα-BGTgroup(P>0.05).(3)Comparedwiththecontrolgroup,thelevelsofIL-1β,IL-6,andTNF-αweresignificantlydecreasedinthepueraringroupandt
