膝关节腔内同种异体脱钙骨复合软骨细胞5c骨髓间充质干细胞组织工程软骨培养的试验研究

《膝关节腔内同种异体脱钙骨复合软骨细胞5c骨髓间充质干细胞组织工程软骨培养的试验研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、TissueEngineeredArticularCartilagewithDecalcifiedBoneMatrixandHomologousChondrocyteand／orBoneMarrowMesenchymalStemCellinRabbits’Knee：AnExperimentalResearchAbstractObjectiveToidentificatethefeasibilityofcultivatingtissueengineeredarticularcartilageinkneecavityanddemonstratetheroleofbonema

2、rrowmesenchymalstemcellinthecultivatingoftissueengineeredcartilage．MaterialandmethodAll90NewZealandwhiterabbits，agefrom6mto8m，weightfrom2．5,4．0kg，male，wererandomlydividedintothreegroups，eachgroupfor30rabbits．GroupA，graftedinthekneewithbonedecalcifiedmatrixonly；10specimensweretakenoutatth

3、etimeof8w，10wand12wrespectivelyforpathologicalobservation．GroupB，graftedinthekneewitllbonedecalcifiedmatrixandhomologousarticularchondrocyte；10specimensweretakenoutatthetimeof8w，10wand12wrespectivelyforpathologicalobservation．GroupC，磐medinthekneewitllbonedecalcifiedmatrixandarticularchon

4、drocyteandbonemarrowmesenchymalstemcell；also10specimensweretakenoutatthetimeof8w，10wand12wrespectivelyforpathologicalobservation．Allthespecimensweretowrapupwithauto-periosteumatthebeginningofgraftingsurgeryusingmicro··surgerytechnology．AllthespecimenswerecarriedoutwithH-·Estainandtoluidi

5、nebluestain，andthenumberofcellswhichistoluidinebluepositiveundertheobservationofinvertedmicroscope(10x40)wererecordedforstatisticanalyzeusingSPSSv14．0．MeasurementdataWeredemonstratedwithrecall士standarddeviationmode，One—wayANOVAandPostHocTests(Dutmett)werecarriedoutforgroupcomparison．5Res

6、ultsGroupC(decalcifiedbonematrixandchondrocyteandbonemarrowmesenehymalstemcell)：Aftercultivatinginthekneecavityfor8weeks，H—Estainshowthatthechondrocytehasbegantoproliferate，andingoodmetabolismstasis，thecellbodiesgrownbigger,karyotinsarereinforced，andalreadyhassecretionofcellmatrix．Atthe8

7、lhweekofgrowth，thespecimensbecamehyalinecartilagesubstance，hascartilagelacuna,andsurroundedbyapondofcellularmatrix，decalcifiedbonematrixstructuredisappeared．Ontoluidinebluestainsamples，groupCat8伍weekshowthesameasH—Estain，themajorityofcellularkaryotinsareindeephyacinthine(