骨髓间充质干细胞及软骨细胞共培养复合同种异体脱钙骨基质修复关.doc

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1、骨髓间充质干细胞及软骨细胞共培养复合同种异体脱钙骨基质修复关作者：冯万文，夏亚一，孙正义，吴萌，王翠芳，党跃修【关键词】骨髓间充质干细胞；软骨细胞；共同培养技术；脱钙骨基质；关节软骨修复Fullthicknessarticularcartilagedefectrepairmentbycocultureofautogenicbonemarrowderivedmesenchymalstemcellswithchondrocytesandimplantationintoallogenicdemineralizedbonematrixinrabbits[Abst

2、ract】AIM：Toinvestigatethefeasibilityofarticularcartilagedefectrepairmentbycocultureofautogenicbonemarrowderivedmesenchymalstemcells(BMSCs)withchondrocytetesandimplantationintoallogenicdemineralizedbonematrix(DBM),evaluatetheoutcomesofrepairmentin。词©!?toprovideatheoreticalbasisfor

3、optimizingcellresourcesforseeding.METHODS：TwopassagedBMSCsandchondrocyteswerecollectedbothataconcentrationof5X109/Landthencoculturedat2*1.ArticularcartilagedefectsinthekneejointsofrabbitsrepairedbythecoculturedcellsseededintoallogenicDBMservedasexperimentaigroupA,bysimpleDBMascon

4、trolgroupBandbynothingascontrolgroupC.Repairedtissueswereevaluatedwithmacroscopicobservation,histologicalscoresandiinmunohistochemicalstainingatweek8and16aftertransplantation.RESULTS：Chondrocytescoculturedbecamerichinextracellularmatrixandproliferatedprompt1y.Theratioofchondrocyt

5、estoBMSCsachievedabove1：1at5~7daftercoculture・IntheexperimentedgroupArepairedtissuesrepresentedhyaline,smoothandflat・Chondrocytesinthezonesofintegratingwithperipheralnativecartilagesweremoremature.InthecontrolgroupBandC,repairedtissueswerefibers・Histologicalscoresindicatedthatexp

6、erimentaigroupAexcelledgroupBandC,andthedifferenceswerestatisticallysignificant(P<；0.01)；differencesbetweengroupBandCwerenotsignificant(P>；0.05).Immunohistochemicalstainingshowedthatcellsinthezonesofrepairedtissueswerelargerinsize,arrangedcolumnnedly,richintypeIIcollagenmatri

7、xandintegratedsatisfactorilywithnativeadjacentcartilagesandsubchondralbonesintheexperimentalgroupAatweek16postoperatively.CONCLUSION：CocultureofautogenicBMSCswithchondrocytescanpromotetheproliferationofchondrocytesandtheproductionofchondralmatrix・Coculturedcellsforseedingcansavea

8、numberofchondrocytes,shortencultureperio