骨髓间充质干细胞和软骨细胞共培养复合同种异体脱钙骨基质修复关节软骨全层缺损

《骨髓间充质干细胞和软骨细胞共培养复合同种异体脱钙骨基质修复关节软骨全层缺损》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、骨髓间充质干细胞和软骨细胞共培养复合同种异体脱钙骨基质修复关节软骨全层缺损作者：冯万文，夏亚一，孙正义，吴萌，王翠芳，党跃修【关键词】骨髓间充质干细胞；软骨细胞；共同培养技术；脱钙骨基质；关节软骨修复Fullthicknessarticularcartilagedefectrepairmentbycocultureofautogenicbonemarrowderivedmesenchymalstemcellswithchondrocytesandimplantationintoallogenicdemineralizedbo

2、nematrixinrabbits【Abstract】AIM:Toinvestigatethefeasibilityofarticularcartilagedefectrepairmentbycocultureofautogenicbonemarrowderivedmesenchymalstemcells(BMSCs)withchondrocytetesandimplantationintoallogenicdemineralizedbonematrix(DBM),evaluatetheoutcomesofrepairmen

3、tinordertoprovideatheoreticalbasisforoptimizingcellresourcesforseeding.METHODS:TwopassagedBMSCsandchondrocyteswerecollectedbothataconcentrationof×109/Landthencoculturedat∶1.Articularcartilagedefectsinthekneejointsofrabbitsrepairedbythecoculturedcellsseededintoallog

4、enicDBMservedasexperimentalgroupA,bysimpleDBMascontrolgroupBandbynothingascontrolgroupC.Repairedtissueswereevaluatedwithmacroscopicobservation,histologicalscoresandimmunohistochemicalstainingatweekand1aftertransplantation.RESULTS:Chondrocytescoculturedbecamerichine

5、xtracellularmatrixandproliferatedpromptly.TheratioofchondrocytestoBMSCsachievedabove1∶1at-daftertheexperimentalgroupArepairedtissuesrepresentedhyaline,smoothandflat.Chondrocytesinthezonesofintegratingwithperipheralnativecartilagesweremoremature.InthecontrolgroupBan

6、dC,repairedtissueswerescoresindicatedthatexperimentalgroupAexcelledgroupBandC,andthedifferenceswerestatisticallysignificant(P).Immunohistochemicalstainingshowedthatcellsinthezonesofrepairedtissueswerelargerinsize,arrangedcolumnnedly,richintypeⅡcollagenmatrixandinte

7、gratedsatisfactorilywithnativeadjacentcartilagesandsubchondralbonesintheexperimentalgroupAatweek1postoperatively.CONCLUSION:CocultureofautogenicBMSCswithchondrocytescanpromotetheproliferationofchondrocytesandtheproductionofchondralmatrix.Coculturedcellsforseedingca

8、nsaveanumberofchondrocytes,shortencultureperiodsandreducesubculturetimes.CoculturedcellsembeddedintoDBMcanrepairarticularcartilagedefectseffectiv