空肠弯曲菌cdta、cdtb、cdtc基因的构建与表达

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1、i1I_j卜．．目录中文摘要⋯⋯000⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯1英文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯s研究论文空肠弯曲菌cdtA、cdtB、cdtC基因构建与表达引言⋯⋯⋯⋯⋯⋯⋯⋯······⋯⋯⋯··⋯⋯·⋯⋯···⋯⋯⋯．．．⋯．．．．．．9第一部分空肠弯曲菌候选疫苗基因的T—A克隆—1上．—j一日IJ吾””””“””“⋯⋯”一一“一”“．．⋯．．．．．．⋯·⋯························12材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯13结果⋯⋯⋯⋯⋯⋯⋯⋯⋯

2、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯19附图⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯20附表⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯28讨论······⋯··⋯⋯⋯·················⋯····⋯⋯⋯····⋯······．·．．．．．．⋯．30小结⋯⋯·······⋯··⋯·········⋯⋯⋯⋯····⋯·············．．⋯．．．⋯．．．．．32参考文献⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯32第二部分空肠弯曲菌cdtA、cdtB、cdtC基因的克隆、序列分析及B细胞表位测—j

3、L．．1一刖舌“”““”““”””“””⋯⋯““．．⋯．．．．．．······⋯⋯⋯···⋯··········35材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯35结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯37附图⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯41讨论···············⋯⋯⋯···⋯⋯⋯·························⋯⋯．．．．．．．．．．．46小结··········⋯⋯⋯⋯⋯··⋯··············⋯···⋯···⋯··．．．．．．⋯．．．．．．．

4、．47参考文献⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯47第三部分空肠弯曲菌cdtA及其高抗原肽段的原核重组质粒的构建及表达．^L—jL·月IJ舌”一“⋯”“”“一”””“一“一⋯⋯⋯．．一”·················⋯⋯··⋯··50材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯51结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯53附图⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯54讨论⋯·····⋯⋯·······⋯······················⋯⋯·········．·．·．．

5、．．．．．．．．⋯56小结············⋯······⋯⋯⋯···⋯·························．·⋯．．．．．．．．．．．．56参考文献⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯57第四部分空肠弯曲菌cdtA、cdtB、cdtC真核表达重组载体的构建与表达前言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯···⋯58日IJ舌⋯“⋯⋯⋯”””””””””””””””⋯⋯⋯一”““”“⋯⋯⋯““58材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯59结果⋯”””⋯”””””””一““一””““·

6、·····⋯·········IO61附图⋯⋯⋯⋯0OO⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯62讨论⋯··⋯⋯⋯········⋯····⋯··························⋯⋯·····⋯·⋯··63／j、结···················⋯·⋯·⋯········一················一················⋯·64参考文献⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯64综述空肠弯曲菌候选疫苗基因及肽疫苗的探索性研究⋯⋯⋯⋯⋯65致谢⋯⋯·························

7、····⋯⋯⋯⋯···⋯⋯····························74个人简历⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯75L中文摘要空肠弯曲菌cdtA、cdtB、cdtC基因构建与表达摘要目前，空肠弯曲菌(Campylobacter．jejuni，C．jejuni)感染是全球范围内急性胃肠炎最常见的病因之一。更具严重后果的是该菌与格林．巴利综合征(Ouillain．Barr6syndrome，GBS)有非常密切的关系，是GBS发生的重要前驱感染因素。但是，由于目前对空肠弯曲菌的遗传学特性、毒力因子、致病作用和空肠

8、弯曲菌诱发GBS的机制尚不完全明了，至今仍缺少有效而可行措施来预防空肠弯曲菌感染引起的腹泻和GBS的发生。为此，本研究针对空肠弯曲菌，选择具有致病性、保守性、免疫原性的基因克隆入