出生后早期接触低剂量速灭威对小鼠神经发育的影响

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1、安擞医科大学硕士学位论文5．结论出生后早期接触低剂量MTMC，对仔鼠躯体感觉运动功能发育的影响至青年期可恢复：虽不引起大脑大体形态的改变，但小鼠成年后的空间记忆能力下降，且大脑皮层突触蛋白GAP-43增加。6．关键词小鼠速灭威神经发育神经行为海马胆碱酯酶GAP．434安徽医科大学硕士学位论文Developmentalneurotoxicityofmiceafterpostnatalexposuretoofm-tolylmethylcarbamateBackgroundAbstraetm-tolylmethylcarbamate(MTMC)isoneofcarbamatespestici

2、de，humansareexposedtoMTMC，fromvariousenvironmentalsources．MTMCisneurotoxicinthatincludepesticidaltoxicology,neuraldevelopmentaltoxicity．RecentlythestudyshowedthatchronicandlowdosageexposuredtoMTMCseemedtobemoreatriskforrecognization，psychologydeficts，developingParkinson’sdiseaseandotherneurodege

3、nerativedisease．ButchronicandlowdosageexposuredtoMTMCthatinducedneuraldevelopmentaltoxicitystillislillknownandunconcern．ObjectiveToexplorethesensitiveneurobehaviorindexsofexposuredtoMTMCinmicebyaseriesmethodsofbehavior,theresultsCanbereferencedinneuraldevelopmentaltoxicityinhuman．Tostudycholines

4、teraseactivityinbrainandtheexpressionofGAP一43．ToinvestigateneuraldevelopmentaltoxicitymechanismofMTMC．MethodsAllmiceexceptcontrolswereinjectedwithdifferentdosesofMTMC(O．05，0．5，5mg·k百1，i．P．)everyotherdayonpostnatalday(PND)3．13，thePBS．andDMSO，treatedmiceservedascontrols．AfterexposuredtoMTMC，observ

5、ephysiologicalgrowth，sensorymovementandloctomotionandharmonyabilityoflittlemousebyaseriesmethodsofdevelopmentalneurobehavior，learningandmemoryabilityinmicebyMorriswatermaze，spontaneousmotoractivitybyholeboardtest，openfield，wiresuspensionandbalancebeamtestdetectbalancecapability,anxietybyblack-wh

6、itealley,安徽医科大学硕士学位论文levelsofAchEweremeasured．TheproteinexpressionofGAP·43wasobservedbyWesternblotting．ThedatawereanalyzedwithSPSS10．0ResultsTherewasasignificantdelayineyeopeninginMTMC—exposuregroupsanderuptingofinferiortoothinthehi曲dosegroupascomparedwithDMSOcontr01．MTMC—exposureinducedreversib

7、leeffectsOilsensofimotoractivitywhichweremeasuredbycliffavoidancetest(PND15)andhorizontalbartask(PND70)．Nosignificantchangesinopen-fieldactivitywereobserved．Significantdifferencesinthetimeinthetargetquadrantandnumberofplatfo