人脑微血管内皮细胞表达cd24的生物学意义的初步研究

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1、万方数据expressioninneuraldifferentiationandneuralmaturation，andneuronaldifferentiationmarkerandknownofit(suchas"Pitx3，TuJl，MAP2)correlation．NeuraldifferentiationinthehighofCD24isidentifiedmatureneuronstypingeffectivedifferentiationmarkers．ForalltypesofnervecellofCD24proteinhasacorrespondingtransl

2、atedbydifferentexpressive[2]inmalignantcellsfoundhighlevelsofCD24moleculesonthesurface，SOitcanknowthelevelintumorgrowtharecloselyrelated．BiologicalfunctionofinbrainmicrovascularendothelialcellshavenotbeenreportedintheliteratureCD24．ThisresearchthroughtheyeasttwohybridexperimentshowedthatCD24an

3、dP190extracellulardomain(1-203AA)proteininteractionexist．AnduseGST-pulldowntofurthervalidatetheCD24interacfionwithP190．Accordingtotheresults，webelievethatCD24proteinmoleculeintheformationofbrainbloodvesselcellsplaysanimportantrole．Inordertogetamoreaccurateresult，wehavedoneaboutP190andstableofC

4、D24proteinmoleculesaffectnormalcelldifferentiation．Toaccumulatedataforthefurtherstudyofthefunctionofbrainbloodvessels．1．Materials(1)ReagentsforcellcultureandRNAi．(2)ReagentsforWesternblotandIF．(3)Reagentsforcellproliferationassayandcellmigrationandcellpermeability．2．Methods(1)Detecttheproteinp

5、rofileofCD24onHBMECbyIFandIFtodetectdistributionofCD24onHBMEC．(2)IPtoidentifyinteractofCD24andP190．(3)WoundscratchassaytodetectcellmigrationandTranswelltodetectpermeabilityofHBMECandwsttodetectcellproliferationafterCD24knockdownandOVerexpression．Resuits1．CD24specificallyexpressedinthecytolymph

6、ofHBMEC．万方数据2．IPidentifyinteractofCD24andP190．3．CellproliferationhavenotchangeaftermodifytheexpressofCD24．TheabilityofcellmigrationhavebecomewakeafterknockdownCD24andbecomestrongafteroverexpressCD24．TheabilityofcellpermeabilityhavebecomestrongafterknockdownCD24andbecomewakeafteroverexpressCD24

7、．ConclusionIPidentifyintracellularinteractionofCD24withP190，CD24stableinterferencemakesHBMECmigrationabilityweak，andmakespermeabilitystrong．HighexpressionofCD24stableenhancedHBMECmigrationdecreasedpermeability．CD24；P190；HBMECKeywords6万方