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ID:32362334
大小:4.61 MB
页数:37页
时间:2019-02-03
《人脑微血管内皮细胞表达cd24的生物学意义的初步研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据expressioninneuraldifferentiationandneuralmaturation,andneuronaldifferentiationmarkerandknownofit(suchas"Pitx3,TuJl,MAP2)correlation.NeuraldifferentiationinthehighofCD24isidentifiedmatureneuronstypingeffectivedifferentiationmarkers.ForalltypesofnervecellofCD24proteinhasacorrespondingtransl
2、atedbydifferentexpressive[2]inmalignantcellsfoundhighlevelsofCD24moleculesonthesurface,SOitcanknowthelevelintumorgrowtharecloselyrelated.BiologicalfunctionofinbrainmicrovascularendothelialcellshavenotbeenreportedintheliteratureCD24.ThisresearchthroughtheyeasttwohybridexperimentshowedthatCD24an
3、dP190extracellulardomain(1-203AA)proteininteractionexist.AnduseGST-pulldowntofurthervalidatetheCD24interacfionwithP190.Accordingtotheresults,webelievethatCD24proteinmoleculeintheformationofbrainbloodvesselcellsplaysanimportantrole.Inordertogetamoreaccurateresult,wehavedoneaboutP190andstableofC
4、D24proteinmoleculesaffectnormalcelldifferentiation.Toaccumulatedataforthefurtherstudyofthefunctionofbrainbloodvessels.1.Materials(1)ReagentsforcellcultureandRNAi.(2)ReagentsforWesternblotandIF.(3)Reagentsforcellproliferationassayandcellmigrationandcellpermeability.2.Methods(1)Detecttheproteinp
5、rofileofCD24onHBMECbyIFandIFtodetectdistributionofCD24onHBMEC.(2)IPtoidentifyinteractofCD24andP190.(3)WoundscratchassaytodetectcellmigrationandTranswelltodetectpermeabilityofHBMECandwsttodetectcellproliferationafterCD24knockdownandOVerexpression.Resuits1.CD24specificallyexpressedinthecytolymph
6、ofHBMEC.万方数据2.IPidentifyinteractofCD24andP190.3.CellproliferationhavenotchangeaftermodifytheexpressofCD24.TheabilityofcellmigrationhavebecomewakeafterknockdownCD24andbecomestrongafteroverexpressCD24.TheabilityofcellpermeabilityhavebecomestrongafterknockdownCD24andbecomewakeafteroverexpressCD24
7、.ConclusionIPidentifyintracellularinteractionofCD24withP190,CD24stableinterferencemakesHBMECmigrationabilityweak,andmakespermeabilitystrong.HighexpressionofCD24stableenhancedHBMECmigrationdecreasedpermeability.CD24;P190;HBMECKeywords6万方
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