reck基因在食管癌中的表达特点及意义

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1、ThesignificanceoftheexpressionofRECKinesophagealcancerMasterdegreecandidate：WangXiaoyanSupervisor：ProfessorXuFeiVice-·supervisor：Vice--ProfessorLiYanMajor：HistologyEmbryologyandHumanAnatomyAbstractEsophagealcancer(EC)drivedfromtheabnormalproliferationofesophagealsquamousorgla

2、ndularepithelium．ECinvolvedinanumberoffactorsandgenesandslowlyevolvedthroughmultiplestages(dysplasia，carcinomainsituandinvasive)．EChavehighincidenceindevelopingcountries，andtheyaremainlysquamouscellcarcinoma，mrbidityandmortalityofinpatientswithECChinaishigherintheworld．Withth

3、edevelopmentofmolecularbiology，themolecularmechanismoftheEChavebeenpaidattention．RecentstudiesfoundthattheRECKgenemayplayanimportantroleinthedevelopmentofEC．Objeetive：TakallashfoundthatRECKisanewmetastasissuppressorgene．ThemechanismmaybethatRECKinhibittumorgrowth，invasionandm

4、etastasisbyinhibitingsecretionandactivityofMMP一2andMMP-9andMTI-MMP．Ithasbeenconfirmedthatanumberoftumormetastasiscloselyrelatedtomutation，deletion，methylationabnormalitiesoftheRECKgene，butstudyaboutrelationofRECKgene’expresstionandtumormetastasisisrare．Sowewanttodiscoverthere

5、lationshipbetweenRECKandEC，andhopetofindnewmoleculartargetsofECbydetectingexpressionoftheRECKgeneinECandesophagealcarcinomacellline．Method：AdjacentnormalmucosaofEC，precanceroustissueandECwereselectedformtheCancerHospitalofAnshanCityandsurgicalresectionspecimenswithoutchemothe

6、rapyandradiotherapyinthepastdecade．ThreecasesoffreshesophagealspecimenstakenfromtheAffiliatedHospitalofDalianMedicalUniversityandwithoutpreoperativeradiotherapyandchemotherapy．Thetwoindividualsesophagealcancercelllines(TE-·1andECA·-109)comefromtheShanghaiCellBankofChineseAcad

7、emyofSciences．Intheoutstudy，WedetectedtheexpressionofRECKinadjacentnormalmucosaofEC，precanceroustissue，andECtissuesbytheparaffintissuemicroarraytechnologyandimmunehistochemistry(IHC)．WedetectedtheexpressionofRECKinthethreecasesfreshspecimensandtwoECcelllinebyimmunocytochemist

8、ry(Jcc)，reversetranscriptionpolymerasechainreaction(RT—PCT)．Thedataw