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ID:32355972
大小:5.42 MB
页数:28页
时间:2019-02-03
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1、ThesignificanceoftheexpressionofRECKinesophagealcancerMasterdegreecandidate:WangXiaoyanSupervisor:ProfessorXuFeiVice-·supervisor:Vice--ProfessorLiYanMajor:HistologyEmbryologyandHumanAnatomyAbstractEsophagealcancer(EC)drivedfromtheabnormalproliferationofesophagealsquamousorgla
2、ndularepithelium.ECinvolvedinanumberoffactorsandgenesandslowlyevolvedthroughmultiplestages(dysplasia,carcinomainsituandinvasive).EChavehighincidenceindevelopingcountries,andtheyaremainlysquamouscellcarcinoma,mrbidityandmortalityofinpatientswithECChinaishigherintheworld.Withth
3、edevelopmentofmolecularbiology,themolecularmechanismoftheEChavebeenpaidattention.RecentstudiesfoundthattheRECKgenemayplayanimportantroleinthedevelopmentofEC.Objeetive:TakallashfoundthatRECKisanewmetastasissuppressorgene.ThemechanismmaybethatRECKinhibittumorgrowth,invasionandm
4、etastasisbyinhibitingsecretionandactivityofMMP一2andMMP-9andMTI-MMP.Ithasbeenconfirmedthatanumberoftumormetastasiscloselyrelatedtomutation,deletion,methylationabnormalitiesoftheRECKgene,butstudyaboutrelationofRECKgene’expresstionandtumormetastasisisrare.Sowewanttodiscoverthere
5、lationshipbetweenRECKandEC,andhopetofindnewmoleculartargetsofECbydetectingexpressionoftheRECKgeneinECandesophagealcarcinomacellline.Method:AdjacentnormalmucosaofEC,precanceroustissueandECwereselectedformtheCancerHospitalofAnshanCityandsurgicalresectionspecimenswithoutchemothe
6、rapyandradiotherapyinthepastdecade.ThreecasesoffreshesophagealspecimenstakenfromtheAffiliatedHospitalofDalianMedicalUniversityandwithoutpreoperativeradiotherapyandchemotherapy.Thetwoindividualsesophagealcancercelllines(TE-·1andECA·-109)comefromtheShanghaiCellBankofChineseAcad
7、emyofSciences.Intheoutstudy,WedetectedtheexpressionofRECKinadjacentnormalmucosaofEC,precanceroustissue,andECtissuesbytheparaffintissuemicroarraytechnologyandimmunehistochemistry(IHC).WedetectedtheexpressionofRECKinthethreecasesfreshspecimensandtwoECcelllinebyimmunocytochemist
8、ry(Jcc),reversetranscriptionpolymerasechainreaction(RT—PCT).Thedataw
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