jnk信号通路对小胶质细胞功能及其表面tnfr的影响

《jnk信号通路对小胶质细胞功能及其表面tnfr的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、TheinfluencesoftheJNKsignalingpathwayonthefunctionofmicrogialandtheexpressionofTNFRMasterdegreecandidate：ZhangWeiSupervisor：ProfessorLiDanqingMajor：InternalMedicineAbstractobjective：Inthepreviousexperiment，wefoundthatBV2cellspresenteddifferentroleswh

2、enculturedinhypoxiastate．Inthisstudy，wewillfurtherexplortheinfluenceoftheJNKsignalingpathwayonthefunctionofmicrogliaandtheexpressionoftumornecrosisfactorreceptor(TNFR)．Methods：BV2celllineandPC12celllinewereusedtostandformicrogliaandneuronsrespectivel

3、yinthisstudy．BV2cellsaredividedinto：normalcontrolgroup(normoxiagroup)，hypoxialhgroup，hypoxiaIh+SP600125group，hypoxia12hgroupandhypoxia12h+SP600125group．Firstly，westernblotwasusedtodetecttheappropriateSP600125concentrationthatblockedtheJNKsignalingpat

4、hway．Then，BV2cellswereculturedinthesituationofhypoxiawithorwithoutthepresenceofSP600125．PC12cellsurvivalratemeasuredbyCCK一8assayinordertoreflecttheJNKpathwayandBV2cellfunction．AttheSalTletime，analysisBV2cellsTNF—areceptorTNFR1andTNFR2expressionbywest

5、ernblot，anddetectinthedifferentfunctionalstatusBV2cellssecretedexpressionofNGF，TNF-a，andIL-1pbyELISA．Results：1．ThenormalcontrolandtheinjuryofPC12groupsurvivalraterespectivelywas100％and52．37％．TheBV2cellscultureinhypoxiaforlh，thePC12cellsurvivalratewas

6、75．96％；TheSP600125CanenhancePCI2cellsurvivalincreasedto81．88％，significantdifference(P<0．01)．WhileBV2cellhypoxia12h，PCI2cellssurvivalrateis36．87％，theSP600125CanincreasePCI2cellssurvivalrateto57．61％，significantdifference(P<0．01)．2．Undernormalcircumstan

7、ce，theBV2cellsurfacehasnoTNFRlexpression，onlyasmallamountofTNFR2expression．TheBV2cellscultureinhypoxiafor1h，theexpressionofTNFR2increased，addingSP600125canfurtherincreasetheexpressionofTNFR2(P<0．05)；Inhypoxiafor12h，theexpressionofTNFRIincreased，addin

8、g3SP600125canfurtherdecreasetheexpressionofTNFRl(P