ages对人神经母细胞瘤细胞的毒性作用与其机制的初步研究

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1、重庆医科大学硕士研究生学位论文THEEFFECTSoFADVANCEDGLYCATIoNENDPRoDUCTSoNHUMANNEURoBASToMACELLLINESH．SY5YANDITSMECHANISMABSTRACTobjective：Toinvestigatetheeffectofagedglycationendproducts(AGEs)onthegrowthstateandtheexpressionofp-amyloidproteinincultredSH-SY5Ycell，furthertoexplorethemechanismoftox

2、icityofAGEsonSH-SY5Y．Tostudytheeffectionofa--lipoicacidonthetoxicityofAGES．Methods：Advancedglycationend-productswerepreparedbyincubationofbovineserumalbumin(BSA)withglucosefor90days．TheculturedSH-SY5Ycellsweredividedintofivegroupsrandomly．Thefirstgroup．thecellsweretreatedbydiffer

3、entconcentrationsofBSA(0、20、40、80、160、320ug／m1)for48hours．Thesecondgroup，thecellsweretreatedbydifferentconcentrationsofBSA(0、20、40、80、160、320ug／m1)for48hoursThethirdgroup．thecellswereinterventedbyconcentrationsofa-LAfor30minutesbeforetreatedbyAGE—BSAof160ug／mlfor48hours．Thefourth

4、group．thecellswereinterventedbyQ·LAofO．1g／1for30minutesbeforetreatedbyAGE—BSAof80ug／mlfor48hours．Thefifthgroupwasthenormalcontrolgroup．toexaminethecells’growthstatewithMTT5重庆医科大学硕士研究生学位论文metabolicrate，LDHleakagerate，andtheaxonallength．ToexaminetheexpressionlevelofAPPmRNAandRAGEmR

5、NAbyRT-PCR．ToexaminetheproteinexpressionofABinSH-SY5YcellsbycellularinmmunohistochemistryResults：(1)Comparingwiththecontrolgroup，treatmentwithconcentrationsofBSAfor48hours，therewerenodifferenceinMTTmetabolicrate，LDHleakagerateandtheaxonallength．therewerenodifferenceoftheexpressio

6、nofA13protein，theexpressionofAPPmRNAandRAGEmRNAinSH—SY5Ycellsbetweenthetreatedgroupandthecontrolgroup．(2)ComparingwiththeBSAgroup，treatmentwithconcentrationsofAGE．BSA48hours，(>40ug／m1)MTTmetabolicratereducedsignificantly,LDHleakagerateincreasedsignificantly,theaxonallengthshorten

7、edsignificantly．therewerenodifferenceoftheexpressionofAPPmRNAinSH·SY5Ycellsbetweenthetreatedgroupandthecontrolgroup．TheexpressionofRAGEmRNAintreatedgroupsincreasedsignificantly．theexpressionofA13proteinincreasedsignificantlycomparedtocontrolgroup．(3)ComparedwiththeAGE—BSA(160ug／m

8、1)group，c【一LAof0．1g／1inhibitedthetoxicit