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ID:32276475
大小:5.38 MB
页数:31页
时间:2019-02-02
《塞来昔布、丁酸钠对结肠癌hct116细胞增殖影响的实验的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、异有统计学意义(P<0.05)。丁酸钠组亦表现相同特征。塞来昔布与丁酸钠联合用药组COX-2mRNA表达量低于单用药组(P<0.05)。与对照组相比,随着塞来昔布浓度的升高,hTERTmRNA表达量逐渐降低,差异有统计学意义(P<0.05)。丁酸钠组亦表现相同特征。塞来昔布与丁酸钠联合用药组hTERTmRNA表达量低于单用药组(P2、CT-116细胞体外生长的机制之一。2.塞来昔布与丁酸钠对结肠癌细胞的增殖抑制作用可能与通过抑制COX-2及端粒酶的表达从而阻滞细胞周期进展有关。关键词:塞来昔布丁酸钠COX.2端粒酶大肠癌2TheStudiestoAnti.TumorEffectanditsMechanismsofNSAIDsCombinedWithsodiumbutyrateonHumanColonCancerCeliLineHCT..116InVitroMasterdegreecandidate:LiuWenXinSupervisorProfessor:WangZhaoHuiMajor3、:InternalMedicineofDigestionAbstractobjective:Tostudytheanti-tumoreffectanditsmechanismsofNSAIDscombinedwithsodiumbutyrateonhumancoloncancercelllineHCT-116invitro.Methods:Wesubculturethecoloncancel"cellstrainHCT一116,adjustcelltologarithmicgrowthperiod.Takelogarithmicgrowthperiodcel4、lsto96-wellplates,makecellconcentrationsreach1.0X104eachhole,100uleachhole,cultivatethreedayslater,addscelecoxibloOumol/L,200umol/L,400umol/L,sodiumbutyrate0.5mmol/L,lmmol/L,2mmol/L,celecoxib100umol/LplugO.5mmol/Lsodiumbutyrate.Aftertreatmentbythedifferentfactors,wecontinuetocultiv5、atecells24h,48h,72h.ThenweuseMTTmethodanalyzetheeffectofeachdrugoncells;applicationflowcytometrydetectcellcycledistribution;applicationRT-PCRdetecttheexpressofCOX一2andhTERTincoloncancercellHCT一116andtheinfluenceofnon··steroidalanti··inflammatorydrugandsodiumbutyratetoexpressofCOX-·6、2andhTEIU’.Results:1.TheMTTmethodshowscelecoxib,sodiumbutyrateinhibitcoloncancercellHCT-116growthsignificantly,witlltheincreaseofconcentrationandtheextensionoftime,theeffectofinhibitionenhancedobviously(P7、iouslythanthataddsthemalone(P8、G0/GlphaseWasincreasedandS
2、CT-116细胞体外生长的机制之一。2.塞来昔布与丁酸钠对结肠癌细胞的增殖抑制作用可能与通过抑制COX-2及端粒酶的表达从而阻滞细胞周期进展有关。关键词:塞来昔布丁酸钠COX.2端粒酶大肠癌2TheStudiestoAnti.TumorEffectanditsMechanismsofNSAIDsCombinedWithsodiumbutyrateonHumanColonCancerCeliLineHCT..116InVitroMasterdegreecandidate:LiuWenXinSupervisorProfessor:WangZhaoHuiMajor
3、:InternalMedicineofDigestionAbstractobjective:Tostudytheanti-tumoreffectanditsmechanismsofNSAIDscombinedwithsodiumbutyrateonhumancoloncancercelllineHCT-116invitro.Methods:Wesubculturethecoloncancel"cellstrainHCT一116,adjustcelltologarithmicgrowthperiod.Takelogarithmicgrowthperiodcel
4、lsto96-wellplates,makecellconcentrationsreach1.0X104eachhole,100uleachhole,cultivatethreedayslater,addscelecoxibloOumol/L,200umol/L,400umol/L,sodiumbutyrate0.5mmol/L,lmmol/L,2mmol/L,celecoxib100umol/LplugO.5mmol/Lsodiumbutyrate.Aftertreatmentbythedifferentfactors,wecontinuetocultiv
5、atecells24h,48h,72h.ThenweuseMTTmethodanalyzetheeffectofeachdrugoncells;applicationflowcytometrydetectcellcycledistribution;applicationRT-PCRdetecttheexpressofCOX一2andhTERTincoloncancercellHCT一116andtheinfluenceofnon··steroidalanti··inflammatorydrugandsodiumbutyratetoexpressofCOX-·
6、2andhTEIU’.Results:1.TheMTTmethodshowscelecoxib,sodiumbutyrateinhibitcoloncancercellHCT-116growthsignificantly,witlltheincreaseofconcentrationandtheextensionoftime,theeffectofinhibitionenhancedobviously(P7、iouslythanthataddsthemalone(P8、G0/GlphaseWasincreasedandS
7、iouslythanthataddsthemalone(P8、G0/GlphaseWasincreasedandS
8、G0/GlphaseWasincreasedandS
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