塞来昔布、丁酸钠对结肠癌hct116细胞增殖影响的实验的的研究

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1、异有统计学意义(P<0．05)。丁酸钠组亦表现相同特征。塞来昔布与丁酸钠联合用药组COX-2mRNA表达量低于单用药组(P<0．05)。与对照组相比，随着塞来昔布浓度的升高，hTERTmRNA表达量逐渐降低，差异有统计学意义(P<0．05)。丁酸钠组亦表现相同特征。塞来昔布与丁酸钠联合用药组hTERTmRNA表达量低于单用药组(P

2、外生长的机制之一。2．塞来昔布与丁酸钠对结肠癌细胞的增殖抑制作用可能与通过抑制COX-2及端粒酶的表达从而阻滞细胞周期进展有关。关键词：塞来昔布丁酸钠COX．2端粒酶大肠癌2TheStudiestoAnti．TumorEffectanditsMechanismsofNSAIDsCombinedWithsodiumbutyrateonHumanColonCancerCeliLineHCT．．116InVitroMasterdegreecandidate：LiuWenXinSupervisorProfessor：WangZhaoHuiMajor：InternalMedicineo

3、fDigestionAbstractobjective：Tostudytheanti-tumoreffectanditsmechanismsofNSAIDscombinedwithsodiumbutyrateonhumancoloncancercelllineHCT-116invitro．Methods：Wesubculturethecoloncancel"cellstrainHCT一116，adjustcelltologarithmicgrowthperiod．Takelogarithmicgrowthperiodcellsto96-wellplates，makecellc

4、oncentrationsreach1．0X104eachhole，100uleachhole，cultivatethreedayslater,addscelecoxibloOumol／L，200umol／L，400umol／L，sodiumbutyrate0．5mmol／L，lmmol／L，2mmol／L，celecoxib100umol／LplugO．5mmol／Lsodiumbutyrate．Aftertreatmentbythedifferentfactors，wecontinuetocultivatecells24h，48h，72h．ThenweuseMTTmeth

5、odanalyzetheeffectofeachdrugoncells；applicationflowcytometrydetectcellcycledistribution；applicationRT-PCRdetecttheexpressofCOX一2andhTERTincoloncancercellHCT一116andtheinfluenceofnon··steroidalanti··inflammatorydrugandsodiumbutyratetoexpressofCOX-·2andhTEIU’．Results：1．TheMTTmethodshowscelecox

6、ib，sodiumbutyrateinhibitcoloncancercellHCT-116growthsignificantly，witlltheincreaseofconcentrationandtheextensionoftime，theeffectofinhibitionenhancedobviously(P

7、cellHCT-116wasinterventionbycelecoxiborsodiumbutyratealone48h，，theratioofG0／G1phaseWasincreasedandtheratioofSphaseWasdecreased．Withdrugconcentrationincreases，theeffectgetmoreobviously．Comparingwithcelecoxiborsodiumbutyratealone，G0／GlphaseWasincreasedandS