欢迎来到天天文库
浏览记录
ID:32227936
大小:1.25 MB
页数:6页
时间:2019-02-01
《骨髓间充质干细胞上清液对大鼠佐剂性关节炎防治作用的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、intracutaneousinjection.Aftersuccessofmodeling,ratsweregivenMSCssupernatanttreatmentandintervention.Duringtheperiod,bodyweight,anklecircumferenceandhindpawvolumeweremeasured.Andratslivingconditionwereobserved.Atthetreatment21stday,rats'limbjointischeckedbyX-rayexaminationand
2、pathologicalexamination.ResultsAfterusingMSCssupernatanttotreatadjuvantarthritisinrats,thejointswellingofMSCsgroupwereobviouslyrelieved,limbX-rayshowedsmootharticularsurfaceofbone,jointspaceemerged.PathologicalmicroscopicexaminationofHEstaininganklejointshowedjointsynovialle
3、sionsandinflammatorycellinfiltrationsignificantlyreduced,andTissueedemawasobviouslyrelieved.InMSCssupernatantinterventiongroup,rats'primaryinflammationandsecondarylesionswerenotobvious,jointX-rayandpathologicalexaminationshowednoobviousabnormalities,weightincreasedwell,hairc
4、olorisbrightafterthetreatmentwithMSCssupernatant.AllvaluesinMSCssupernatantinterventiongroupwereclosedtonormalsalinecontrolgroup.Comparedwithmodelgroup,thedifferencesofrats'weight,pawvolumeandanklecircumferencehadstatisticallysignificant(P<0.05).ButinL-DMEMculturesolutiontre
5、atmentgroup,thedifferencesofallvaluescomparedwithmodelgroup,hadnostatisticallysignificant(P>0.05).ConclusionsThetreatmentofMSCssupernatantcanrelievejointswellingofAArats,andhaveaneffecttopreventAA'soccurring.ThemechanismsofactionmaybetherewasSolublefactorssecretedbyMSCsinMSC
6、ssupernatant,thespecificingredientswereneedtobefurtherinvestigated.Marrowmesenchymalstemcells;supernatant;adjuvantarthritis;treatment;Intervention七纯亡,适模后关节肿胀且进j9长明显小于正{;3d右足肿胀达_4-0.142.52_~0.07~2.16~2522孑L-DMEM:改变,关寺肢双侧足剐l修复,关j(1)正常组;(21(1》~ormalgr()L292-294.tckSC,etal4(5411
7、):ILneS,etIclinical
此文档下载收益归作者所有