循环细胞膜微粒对缺血性卒中患者内皮细胞功能的影响

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1、万方数据2012级硕士研究生学位论文循环细胞膜微粒对缺血性卒中患者内皮细胞功能的影响EFFECTSOFCIRCULATIONMICROPARTICLESONENDOTHELIALCELLSABSTRACTBackgroundAccordingtothesurveyofworldhealthorganization(WHO)，cerebrovasculardiseaseisoneofthethreemajordiseasesthatresultedinthedeathsofhuman，isalsooftheworld’Sfour

2、thlargestdiseaseleadingtoloselaborability．Thereareallkindsofbiochemicalmarkerscanbeusedtodetectpathologicalstate，suchasinfections，diabetes，anddyslipidemia，buttherewasnospecificbloodbiochemicalmarkerscanbeusedinthemonitoringofcerebrovasculardisease．Microparticleisthe

3、capsulebubblesofffromvariouscellsurfacesinthecirculationsystemwithdiameterbetweenO．1and1um．Peripheralcirculationmicroparticlescanbeproducedbyvarioustypesofcells，suchasplatelets，redbloodcells，endothelialcellsandsmoothmusclecells，andSOon．Manystudiesshowedthatthemembra

4、nesurfaceofmicroparticlescarryspecificantigenandCDmoleculesofitsparentcell．Previousstudiesfoundthattheamountandtypeofcircularmembranemicroparticleiscloselyrelatedtomanydiseases，suchashighbloodpressure，coronaryheartdisease，atherosclerosisandmanyotherdiseases．Atthesam

5、etime，microparticlesarecloselyrelatedtoinflammatoryresponse，apoptosisandotherpathological4万方数据2012级硕士研究生学位论文循环细胞膜微粒对缺血性卒中患者内皮细胞功能的影响physiologicalprocess．Endothelialmicroparticlesoriginatefromendothelialcells，alsoactasasymbolofreflectendothelialcellfunction．Objective

6、Inthisexperiment，weusethemethodofflowcytometry，toinvestigatecirculatinglevelsandantigenexpressionofmicroparticlesinischemiccerebrovasculardisease；alsotoexplorethecorrelationbetweenischemiccerebrovasculardiseaseandmicroparticleslevel．Tofurtherstudyonthemicroparticle’

7、Seffectsonvascularendothelialcellfunction，accordinglytofurtherexploretheroleofmicroparticlesinmechanismofischemiccerebrovasculardisease．MaterialandmethodsInthisexperiment，circularmicroparticlesseparationextractingfromvenousbloodofpatientswiththefirstoccurrenceofisch

8、emicstrokeisactingastheresearchobject．Atthesametime，circularmicroparticlesextractingfromvenousbloodofthehealthyindividualsforphysicalexami