间充质干细胞尿路上皮细胞—脱细胞基质修复尿道缺损

《间充质干细胞尿路上皮细胞—脱细胞基质修复尿道缺损》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、间充质干细胞/尿路上皮细胞—脱细胞基质修复尿道缺损冯春祥范应中孙生安李泸平张胜利李浩王文举摘要：目的：对间充质干细胞—基质复合材料、尿路上皮细胞—基质复合材料与单纯脱细胞基质材料修复尿道缺损效果的差异进行了初步探讨。方法：取离体培养第4代尿路上皮细胞和骨髓间充质干细胞（BMSC）做为种子细胞，行5-溴脱氧尿嘧啶核苷(BdrU)标记后接种至脱细胞基质。分别将复合有上皮细胞、BMSC和空白的基质材料移植到尿道缺损动物模型体内，标记为A、B、C组。术后第1、2、4、12周采集动物修复尿道，行组织学观察。术后12周对实验动物行逆行膀胱尿道造影检查。结果：第1周，A、B两组可

2、见稀疏的单层上皮样细胞覆盖，但A组细胞形状较规则。第2周，A、B两组大仍是单层尿道上皮覆盖。A组上皮细胞的致密度较高。第4周，A、B两组上皮细胞分布与正常组织较接近，但A组细胞分层较好。第12周，各组新生尿道与正常组织已明显区分。C组术后并发症发生率为80%，高于另外两组。结论：1.BMSC或尿路上皮细胞复合脱细胞外基质移植到机体内可有效修复缺损尿道，并且效果优于单纯脱细胞外基质材料。2.接种至第4周，发现尿路上皮细胞复合脱细胞基质材料修复尿道效果较BMSC好。关键词：间充质干细胞尿路上皮细胞脱细胞基质尿道缺损修复UrethralReconstructionbyMe

3、senchymalStemcell/Urothelium-AcellularExtracellularMatrixFENGChun-xiang,FANYing-zhong,SUNSheng-an,LILu-ping,ZhangSheng-li,LiHao,WangWen-junObjective：Comparingtherepairresultsdifference,amongstemcell-acellularextracellularmatrix、urothelium-acellularextracellularmatrixandacellularextrace

4、llularmatrix.Providetheoreticalbasisforthechoosingofurethraltissueengineeringseedcells.Methods:urethroplastywereperformedwithacellularextracellularmatrix(groupC),acellularextracellularmatrixseededwithurothelium(groupB),acellularextracellularmatrixwasseededwithBMSC(groupC).Thenewbornure

5、thraltissuewascutregularly(in1、2、4、12waftersurgery)forhistological、immunochistochmeistryexaminationtocomparetheirdifferences.CellhadbeenpreformedBrdUlabelbeforeseedingintogroupAandgroupB.Weevaluatedtheregenerationbyurethrographyin12w.Results:In1w,groupA、groupBcouldbeseenthinlayerurothe

6、liumcoverageinrepairregion.HowevermostregionofthegroupAcouldbeseencoveredbyurotheliumjunction.;in2w,groupAandgroupBcouldbeseenthesimilaruorhteliumdistribution,butgroupBwasbetterinthedensityofurothelium.In4w,theurotheliumofgroupAandBwasclosetonormal,andthedistributionofgroupAwasbetterth

7、angroupB.In12w，theurotheliumdistributioninrepairregionofeachgroupwasclosetonormal.TheincidenceofpostoperativecomplicationsingroupCwas80%,whichwashigherthanothers.Conclusions:1.BMSCorurothelium-acellularextracellularmatrixcaneffectivelyrepairtheurethraldefection,andtheresultofrepairis