临床医学毕业论文生胃酮对大鼠颈动脉损伤后新生内膜过度增殖的影响

《临床医学毕业论文生胃酮对大鼠颈动脉损伤后新生内膜过度增殖的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、生胃酮对大鼠颈动脉损伤后新生内膜过度增殖的影响姓名：2014年6月25日作者：宋明宝，于学军，崔馨，陈剑飞，卢来春，赵刚，于世勇，董红梅，黄岚【摘要】0的：观察生胃酮对大鼠颈动脉损伤后新生内膜过度增殖的影响。方法：采用组织贴块法培养大鼠主动脉平滑肌细胞，Westernblot检测生胃酮对大鼠主动脉平滑肌细胞缝隙连接蛋白43表达的影响。采用球囊损伤建立大鼠颈动脉损伤模型，对照组给予生理盐水2mL、生胃酮组给予生胃酮3mg/kg腹腔注射，1次/d,HE染色观察新生内膜形成情况，免疫荧光染色检测新生内膜屮缝隙连接蛋白43的表达，伊文思蓝DAPI双染色以评价新生内膜形成情况。结果：所培养

2、的细胞抗SMaactin免疫荧光染色阳性。50(imol/L生胃酮处理平滑肌细胞24h后对缝隙连接蛋白43的表达无影响(0.85士0.06vs0.83±0.03,n=3,P0.05)。球囊损伤大鼠颈动脉14d后可见血管管腔狭窄、新生内膜增生明显。免疫荧光染色显示形成的新生内膜屮缝隙连接蛋內43表达丰富。经生H酮干预后新生内膜增生明显减轻，新生内膜细胞计数显著低于对照组(89土28.40vs236士15.04,n=5,P0.01)。结论:缝隙连接在血管损伤后新生内膜形成过程中扮演重耍角色，而缝隙连接阻断剂生胄酮能抑制新生内膜过度增生。【关键词】新生内膜;血管;缝隙连接;生胃酮;人鼠

3、[Abstract]Objective:Toobservetheeffectofcarbenoxoloneonneointimalhyperplasiaafterratcarotidballooninjury.Methods:RataorticSMCs(RASMCs)wereculturedbyexplantedrataorticwalltissueandidentifiedwithcellimmunofluorescencestainingforSMaactin.TheexpressionofCx43inRASMCswhichweretreatedwithcarbenoxolo

4、neataconcentrationof50pmol/Lfor24hwasdetectedwithwesternblot.Themodelofvascularinjurywasestablishedwithratcarotidballooninjury.Andanimalswereadministratedwithintraperitonealinjectionsofcarbenoxolone[3mg/(kgd)]inthecarbenoxolonegrouporwithsaline(2mL/d)inthecontrolgroupfor2weeksaftercarotidball

5、ooninjury.After2weeks,HEstainingandDAPIEvensbluedoublestainingwereappliedtoevaluatetheneointimalformationoftargetedvessels.AndcellimmunofluorescencestainingwasusedtodetectproteinCx43expressionontargetedvessels.Results:RASCMCswereculturedsuccessfullywithpositiveimmunofluorescencestainingforSMa

6、actin.Inthisexperiment，wefoundthatcarbenoxolonecouldntinducetheexpressionofCx43proteininRASMCs(0.85土0.06vs0.83土0.03,n=3,P0.05).Twoweeksaftercarotidballooninjury，carbenoxolonecouldsignificantlyreducetheneointimalformationandthestenosisofbloodvessellumen.Whennucleinumberofneointimawasusedtoeval

7、uatetheneointimalformation，resultsuggestedthatnucleinumberofneointimaincarbenoxolonegroupwassignificantlylowerthanthatinthecontrolgroup(89士28.40vs236土15.04，n=5，P0.01).TheexpressionofCx43proteininneointimawasabundant.Conclusions:Gapjunctionspl